Protection Against Apoptosis by the Vaccinia Virus SPI-2 (B13R) Gene Product

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1996 Sep 1

PMID 8709286

Citations 58

Authors

M Dobbelstein

T Shenk

Affiliations

Soon will be listed here.

Abstract

Vaccinia virus contains a gene, termed SPI-2 or B13R, that is closely related in its sequence to a potent inhibitor of apoptosis from cowpox virus (crmA). Infection by vaccinia virus protects HeLa cells against apoptosis that is induced by an immunoglobulin M antibody against the fas receptor or by tumor necrosis factor alpha. This effect is profoundly reduced when the SPI-2 gene is deleted. The SPI-2 gene, when transiently expressed in these cells, can also protect against apoptosis mediated by these agents. Given the similarity to crmA, it seems likely that SPI-2 functions in an analogous fashion, inhibiting the activity of ICE protease family members and blocking the onset of apoptosis.

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References

Smith G, Howard S, Chan Y . Vaccinia virus encodes a family of genes with homology to serine proteinase inhibitors. J Gen Virol. 1989; 70 ( Pt 9):2333-43. DOI: 10.1099/0022-1317-70-9-2333. View

Kotwal G, Moss B . Vaccinia virus encodes two proteins that are structurally related to members of the plasma serine protease inhibitor superfamily. J Virol. 1989; 63(2):600-6. PMC: 247729. DOI: 10.1128/JVI.63.2.600-606.1989. View

Zhou J, Crawford L, McLean L, Sun X, Stanley M, Almond N . Increased antibody responses to human papillomavirus type 16 L1 protein expressed by recombinant vaccinia virus lacking serine protease inhibitor genes. J Gen Virol. 1990; 71 ( Pt 9):2185-90. DOI: 10.1099/0022-1317-71-9-2185. View

Gregory C, Dive C, Henderson S, Smith C, Williams G, Gordon J . Activation of Epstein-Barr virus latent genes protects human B cells from death by apoptosis. Nature. 1991; 349(6310):612-4. DOI: 10.1038/349612a0. View

Buller R, Palumbo G . Poxvirus pathogenesis. Microbiol Rev. 1991; 55(1):80-122. PMC: 372802. DOI: 10.1128/mr.55.1.80-122.1991. View

Moss B . Vaccinia virus: a tool for research and vaccine development. Science. 1991; 252(5013):1662-7. DOI: 10.1126/science.2047875. View

Henderson S, Rowe M, Gregory C, Croom-Carter D, Wang F, Longnecker R . Induction of bcl-2 expression by Epstein-Barr virus latent membrane protein 1 protects infected B cells from programmed cell death. Cell. 1991; 65(7):1107-15. DOI: 10.1016/0092-8674(91)90007-l. View

Turner P, Moyer R . An orthopoxvirus serpinlike gene controls the ability of infected cells to fuse. J Virol. 1992; 66(4):2076-85. PMC: 288998. DOI: 10.1128/JVI.66.4.2076-2085.1992. View

Dobbelstein M, Arthur A, Dehde S, Van Zee K, Dickmanns A, Fanning E . Intracistronic complementation reveals a new function of SV40 T antigen that co-operates with Rb and p53 binding to stimulate DNA synthesis in quiescent cells. Oncogene. 1992; 7(5):837-47. View

10.

Ray C, Black R, Kronheim S, Greenstreet T, Sleath P, Salvesen G . Viral inhibition of inflammation: cowpox virus encodes an inhibitor of the interleukin-1 beta converting enzyme. Cell. 1992; 69(4):597-604. DOI: 10.1016/0092-8674(92)90223-y. View

11.

White E, Sabbatini P, Debbas M, Wold W, Kusher D, Gooding L . The 19-kilodalton adenovirus E1B transforming protein inhibits programmed cell death and prevents cytolysis by tumor necrosis factor alpha. Mol Cell Biol. 1992; 12(6):2570-80. PMC: 364450. DOI: 10.1128/mcb.12.6.2570-2580.1992. View

12.

Spriggs M, Hruby D, Maliszewski C, Pickup D, Sims J, Buller R . Vaccinia and cowpox viruses encode a novel secreted interleukin-1-binding protein. Cell. 1992; 71(1):145-52. DOI: 10.1016/0092-8674(92)90273-f. View

13.

Crook N, Clem R, Miller L . An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif. J Virol. 1993; 67(4):2168-74. PMC: 240327. DOI: 10.1128/JVI.67.4.2168-2174.1993. View

14.

Miura M, Zhu H, Rotello R, Hartwieg E, Yuan J . Induction of apoptosis in fibroblasts by IL-1 beta-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3. Cell. 1993; 75(4):653-60. DOI: 10.1016/0092-8674(93)90486-a. View

15.

Gagliardini V, Fernandez P, Lee R, Drexler H, Rotello R, Fishman M . Prevention of vertebrate neuronal death by the crmA gene. Science. 1994; 263(5148):826-8. DOI: 10.1126/science.8303301. View

16.

Palumbo G, Buller R, Glasgow W . Multigenic evasion of inflammation by poxviruses. J Virol. 1994; 68(3):1737-49. PMC: 236634. DOI: 10.1128/JVI.68.3.1737-1749.1994. View

17.

Gonzalo J, Gonzalez-Garcia A, Kalland T, Hedlung G, Martinez C, Kroemer G . Linomide inhibits programmed cell death of peripheral T cells in vivo. Eur J Immunol. 1994; 24(1):48-52. DOI: 10.1002/eji.1830240108. View

18.

Komiyama T, Ray C, Pickup D, Howard A, Thornberry N, Peterson E . Inhibition of interleukin-1 beta converting enzyme by the cowpox virus serpin CrmA. An example of cross-class inhibition. J Biol Chem. 1994; 269(30):19331-7. View

19.

Ink B, GILBERT C, Evan G . Delay of vaccinia virus-induced apoptosis in nonpermissive Chinese hamster ovary cells by the cowpox virus CHOhr and adenovirus E1B 19K genes. J Virol. 1995; 69(2):661-8. PMC: 188626. DOI: 10.1128/JVI.69.2.661-668.1995. View

20.

Kettle S, Blake N, Law K, Smith G . Vaccinia virus serpins B13R (SPI-2) and B22R (SPI-1) encode M(r) 38.5 and 40K, intracellular polypeptides that do not affect virus virulence in a murine intranasal model. Virology. 1995; 206(1):136-47. DOI: 10.1016/s0042-6822(95)80028-x. View