Ability of Spleen, Peritoneal Cavity, and Lymph Node B Cells to Reconstitute Serum Immunoglobulin in SCID Mice

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1996 May 1

PMID 8707345

Citations 4

Authors

J Riggs

R Stowers

Affiliations

Soon will be listed here.

Abstract

The impact of intrinsic B lymphocyte heterogeneity and of microenvironmental influences on serum immunoglobulin production by B cells was examined by intravenous (i.v.) and intraperitoneal (i.p.) transfer of BALB/c and BALB.xid (X-chromosome-linked immunedefective; XID) lymph node (LN), splenic (SP) and peritoneal cavity (PerC) cells into severe-combined immune-defective (SCID) mice. The results indicate that each B-cell source restores all immunoglobulin classes within 5 weeks of transfer, the rates for each isotype, however, differ between the B-cell sources. Serum IgM levels were restored most rapidly by PerC cell transfer, followed by SP and LN cell transfer. In addition, normal immunoglobulin levels were reached in the absence of complete lymphoid reconstitution. Serum immunoglobulin phenotypes characteristic of the donor strain, e.g. reduced IgM and IgG3 production by XID B cells, were maintained after transfer into the SCID recipient. Microenvironmental influences were indicated by reduced immunoglobulin production after i.p. transfer and after i.v. transfer into irradiated SCID recipients. The data show that both B-cell type and microenvironment play significant roles in generating the heterogeneous pool of B cells required for humoral immunity.

Citing Articles

Age-dependent increase of peritoneal B-1b B cells in SCID mice.

Hinkley K, Chiasson R, Prior T, Riggs J Immunology. 2002; 105(2):196-203.

PMID: 11872095 PMC: 1782648. DOI: 10.1046/j.1365-2567.2002.01360.x.

DBA/2J (Mls-1a) B-cell differentiation in BALB.xid recipients.

Tocce K, Suppiah K, Rago C, Liberchuk R, Duffy K, Riggs J Immunology. 2000; 99(1):147-52.

PMID: 10651953 PMC: 2327133. DOI: 10.1046/j.1365-2567.2000.00929.x.

Increased serum IgG1 levels and reduced numbers of B-1 B cells in DBA/2J mice.

Tocce K, MASTERS G, Rago C, Prior L, Suppiah K, Leary S Immunology. 1998; 94(3):403-9.

PMID: 9767424 PMC: 1364260. DOI: 10.1046/j.1365-2567.1998.00531.x.

Recipient age determines the success of intraperitoneal transplantation of peritoneal cavity B cells.

Julius Jr P, Kaga M, Palmer Y, Vyas V, Prior L, Delice D Immunology. 1997; 91(3):383-90.

PMID: 9301527 PMC: 1364007. DOI: 10.1046/j.1365-2567.1997.00270.x.

References

Bosma G, CUSTER R, Bosma M . A severe combined immunodeficiency mutation in the mouse. Nature. 1983; 301(5900):527-30. DOI: 10.1038/301527a0. View

Wortis H, Teutsch M, Higer M, Zheng J, Parker D . B-cell activation by crosslinking of surface IgM or ligation of CD40 involves alternative signal pathways and results in different B-cell phenotypes. Proc Natl Acad Sci U S A. 1995; 92(8):3348-52. PMC: 42163. DOI: 10.1073/pnas.92.8.3348. View

Dorshkind K, Pollack S, Bosma M, Phillips R . Natural killer (NK) cells are present in mice with severe combined immunodeficiency (scid). J Immunol. 1985; 134(6):3798-801. View

Freitas A, Rocha B, COUTINHO A . Two major classes of mitogen-reactive B lymphocytes defined by life span. J Immunol. 1986; 136(2):466-9. View

Mosmann T, Cherwinski H, Bond M, Giedlin M, Coffman R . Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986; 136(7):2348-57. View

Pereira P, Forni L, Larsson E, Cooper M, Heusser C, Coutinho A . Autonomous activation of B and T cells in antigen-free mice. Eur J Immunol. 1986; 16(6):685-8. DOI: 10.1002/eji.1830160616. View

Stall A, Lalor P, Sidman C, Moore W, Parks D, Herzenberg L . The Ly-1 B cell lineage. Immunol Rev. 1986; 93:81-102. DOI: 10.1111/j.1600-065x.1986.tb01503.x. View

Reininger L, Ollier P, Poncet P, Kaushik A, Jaton J . Novel V genes encode virtually identical variable regions of six murine monoclonal anti-bromelain-treated red blood cell autoantibodies. J Immunol. 1987; 138(1):316-23. View

Forster I, Rajewsky K . Expansion and functional activity of Ly-1+ B cells upon transfer of peritoneal cells into allotype-congenic, newborn mice. Eur J Immunol. 1987; 17(4):521-8. DOI: 10.1002/eji.1830170414. View

10.

Klinman N, Linton P . The clonotype repertoire of B cell subpopulations. Adv Immunol. 1988; 42:1-93. DOI: 10.1016/s0065-2776(08)60842-1. View

11.

von Boehmer H . The developmental biology of T lymphocytes. Annu Rev Immunol. 1988; 6:309-26. DOI: 10.1146/annurev.iy.06.040188.001521. View

12.

Lalor P, Herzenberg L, Adams S, Stall A . Feedback regulation of murine Ly-1 B cell development. Eur J Immunol. 1989; 19(3):507-13. DOI: 10.1002/eji.1830190315. View

13.

Carroll A, Hardy R, Bosma M . Occurrence of mature B (IgM+, B220+) and T (CD3+) lymphocytes in scid mice. J Immunol. 1989; 143(4):1087-93. View

14.

Riggs J, Stowers R, Mosier D . The immunoglobulin allotype contributed by peritoneal cavity B cells dominates in SCID mice reconstituted with allotype-disparate mixtures of splenic and peritoneal cavity B cells. J Exp Med. 1990; 172(2):475-85. PMC: 2188330. DOI: 10.1084/jem.172.2.475. View

15.

Fulop G, Phillips R . The scid mutation in mice causes a general defect in DNA repair. Nature. 1990; 347(6292):479-82. DOI: 10.1038/347479a0. View

16.

McIntyre T, Holmes K, Steinberg A, Kastner D . CD5+ peritoneal B cells express high levels of membrane, but not secretory, C mu mRNA. J Immunol. 1991; 146(10):3639-45. View

17.

Chesebro B, Metzger H . Affinity labeling of a phosphorylcholine binding mouse myeloma protein. Biochemistry. 1972; 11(5):766-71. DOI: 10.1021/bi00755a014. View

18.

INMAN J . Thymus-independent antigens: the preparation of covalent, hapten-ficoll conjugates. J Immunol. 1975; 114(2 Pt 1):704-9. View

19.

Perlmutter R, Nahm M, STEIN K, Slack J, Zitron I, Paul W . Immunoglobulin subclass-specific immunodeficiency in mice with an X-linked B-lymphocyte defect. J Exp Med. 1979; 149(4):993-8. PMC: 2184855. DOI: 10.1084/jem.149.4.993. View

20.

Mond J, SCHER I, Cossman J, Kessler S, Mongini P, Hansen C . Role of the thymus in directing the development of a subset of B lymphocytes. J Exp Med. 1982; 155(3):924-36. PMC: 2186612. DOI: 10.1084/jem.155.3.924. View