Flow Cytometric Analysis of Cytokine Receptors on Human Langerhans' Cells. Changes Observed After Short-term Culture

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1996 Feb 1

PMID 8698397

Citations 12

Authors

A Larregina

A Morelli

E Kolkowski

L Fainboim

Affiliations

Soon will be listed here.

Abstract

It is well established that granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-1 and tumour necrosis factor-alpha (TNF-alpha) are involved in Langerhans' cell (LC) development and dendritic cell traffic. However, little is known about the pattern of cytokine receptors on human LC and their modulation during different stages of maturation. The expression of cytokine receptors was studied by flow cytometry on both freshly isolated LC (fLC) and 72-hr cultured LC (cLC). Epidermal cell suspensions enriched in LC were obtained after skin trypsinization and Ficoll-Hypaque gradient. LC were identified by their CD1a positivity. Although the majority of fLC were positive for the alpha chain of GM-CSF receptor (GM-CSFR), the beta chain of GM-CSFR was detected only on 15% of CD1a+ cells. fLC were also positive for IL-1 receptor (IL-1R) type 1, IL-1R type 2, 75,000 molecular weight TNF receptor (TNFR) and interferon-gamma receptor (IFN-gamma R). IL-6R and its transducing signal gp130 were present in a subset of fLC. Granulocyte colony-stimulating factor receptor (G-CSFR), macrophage colony-stimulating factor receptor (M-CSFR), the alpha and beta chain of IL-2R, IL-4R, IL-7R, IL-8R and 55,000 molecular weight TNFR were not detected on fLC. After culture, LC up-regulated the expression of both the alpha and beta chains of GM-CSFR, IL-1R type 2, alpha and beta chains of IL-2R, IL-6R and gp130. In contrast, IL-1R type 1 and 75,000 molecular weight TNFR were down-modulated and the expression of IFN-gamma R was not affected by culture. These results suggest that LC undergo changes in the cytokine receptor repertory during in vitro maturation.

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References

Steiner G, Tschachler E, Tani M, Malek T, Shevach E, Holter W . Interleukin 2 receptors on cultured murine epidermal Langerhans cells. J Immunol. 1986; 137(1):155-9. View

Kaplan G, Walsh G, Guido L, Meyn P, Burkhardt R, Abalos R . Novel responses of human skin to intradermal recombinant granulocyte/macrophage-colony-stimulating factor: Langerhans cell recruitment, keratinocyte growth, and enhanced wound healing. J Exp Med. 1992; 175(6):1717-28. PMC: 2119267. DOI: 10.1084/jem.175.6.1717. View

Heufler C, Koch F, Schuler G . Granulocyte/macrophage colony-stimulating factor and interleukin 1 mediate the maturation of murine epidermal Langerhans cells into potent immunostimulatory dendritic cells. J Exp Med. 1988; 167(2):700-5. PMC: 2188828. DOI: 10.1084/jem.167.2.700. View

Romani N, Lenz A, Glassel H, STOSSEL H, Stanzl U, Majdic O . Cultured human Langerhans cells resemble lymphoid dendritic cells in phenotype and function. J Invest Dermatol. 1989; 93(5):600-9. DOI: 10.1111/1523-1747.ep12319727. View

MacPherson G, Fossum S, Harrison B . Properties of lymph-borne (veiled) dendritic cells in culture. II. Expression of the IL-2 receptor: role of GM-CSF. Immunology. 1989; 68(1):108-13. PMC: 1385513. View

Gearing D, King J, Gough N, Nicola N . Expression cloning of a receptor for human granulocyte-macrophage colony-stimulating factor. EMBO J. 1989; 8(12):3667-76. PMC: 402049. DOI: 10.1002/j.1460-2075.1989.tb08541.x. View

Koch F, Heufler C, Kampgen E, Schneeweiss D, Bock G, Schuler G . Tumor necrosis factor alpha maintains the viability of murine epidermal Langerhans cells in culture, but in contrast to granulocyte/macrophage colony-stimulating factor, without inducing their functional maturation. J Exp Med. 1990; 171(1):159-71. PMC: 2187649. DOI: 10.1084/jem.171.1.159. View

Teunissen M, Wormmeester J, Krieg S, Peters P, Vogels I, Kapsenberg M . Human epidermal Langerhans cells undergo profound morphologic and phenotypical changes during in vitro culture. J Invest Dermatol. 1990; 94(2):166-73. DOI: 10.1111/1523-1747.ep12874439. View

Miyajima A, Kitamura T, Harada N, Yokota T, Arai K . Cytokine receptors and signal transduction. Annu Rev Immunol. 1992; 10:295-331. DOI: 10.1146/annurev.iy.10.040192.001455. View

10.

Takeshita T, Asao H, Ohtani K, Ishii N, Kumaki S, Tanaka N . Cloning of the gamma chain of the human IL-2 receptor. Science. 1992; 257(5068):379-82. DOI: 10.1126/science.1631559. View

11.

Tartaglia L, Goeddel D . Two TNF receptors. Immunol Today. 1992; 13(5):151-3. DOI: 10.1016/0167-5699(92)90116-O. View

12.

Watanabe Y, Kitamura T, Hayashida K, Miyajima A . Monoclonal antibody against the common beta subunit (beta c) of the human interleukin-3 (IL-3), IL-5, and granulocyte-macrophage colony-stimulating factor receptors shows upregulation of beta c by IL-1 and tumor necrosis factor-alpha. Blood. 1992; 80(9):2215-20. View

13.

Matsue H, Cruz Jr P, Bergstresser P, Takashima A . Langerhans cells are the major source of mRNA for IL-1 beta and MIP-1 alpha among unstimulated mouse epidermal cells. J Invest Dermatol. 1992; 99(5):537-41. DOI: 10.1111/1523-1747.ep12667296. View

14.

Caux C, Dezutter-Dambuyant C, Schmitt D, Banchereau J . GM-CSF and TNF-alpha cooperate in the generation of dendritic Langerhans cells. Nature. 1992; 360(6401):258-61. DOI: 10.1038/360258a0. View

15.

Tazi A, Bouchonnet F, Grandsaigne M, Boumsell L, HANCE A, Soler P . Evidence that granulocyte macrophage-colony-stimulating factor regulates the distribution and differentiated state of dendritic cells/Langerhans cells in human lung and lung cancers. J Clin Invest. 1993; 91(2):566-76. PMC: 287979. DOI: 10.1172/JCI116236. View

16.

Enk A, Angeloni V, Udey M, Katz S . An essential role for Langerhans cell-derived IL-1 beta in the initiation of primary immune responses in skin. J Immunol. 1993; 150(9):3698-704. View

17.

Colotta F, Re F, Muzio M, Bertini R, Polentarutti N, Sironi M . Interleukin-1 type II receptor: a decoy target for IL-1 that is regulated by IL-4. Science. 1993; 261(5120):472-5. DOI: 10.1126/science.8332913. View

18.

Ioffreda M, Whitaker D, Murphy G . Mast cell degranulation upregulates alpha 6 integrins on epidermal Langerhans cells. J Invest Dermatol. 1993; 101(2):150-4. DOI: 10.1111/1523-1747.ep12363632. View

19.

Knight S, Stagg A . Antigen-presenting cell types. Curr Opin Immunol. 1993; 5(3):374-82. DOI: 10.1016/0952-7915(93)90056-x. View

20.

Divaris N, Kay C, Carsons S . Mechanisms of tumor necrosis factor-granulocyte-macrophage colony-stimulating factor-induced dendritic cell development. Blood. 1993; 82(10):3019-28. View