Rapid Tube Test for Detecting Fungal Cellulase Production

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1977 Apr 1

PMID 869542

Citations 11

Authors

R E Smith

Affiliations

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Abstract

A simple procedure is described for the detection of cellulolytic activity in fungi, utilizing dyed cellulose powder as substrate. The powder, incorporated into a basal medium containing agar, mineral salts, and growth factors, is layered onto basal medium minus cellulose. Cellulase production results in release of the dye which diffuses into the lower layer.

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References

Coutts A, Smith R . Factors influencing the production of cellulases by Sporotrichum thermophile. Appl Environ Microbiol. 1976; 31(6):819-25. PMC: 169840. DOI: 10.1128/aem.31.6.819-825.1976. View

Poincelot R, Day P . Simple dye release assay for determining cellulolytic activity of fungi. Appl Microbiol. 1972; 23(5):875-9. PMC: 380464. DOI: 10.1128/am.23.5.875-879.1972. View

Fernley H . The use of reactive dyestuffs in enzymology: new substrates for cellulolytic enzymes. Biochem J. 1963; 87:90-5. PMC: 1276844. DOI: 10.1042/bj0870090. View

Rautela G, Cowling E . Simple cultural test for relative cellulolytic activity of fungi. Appl Microbiol. 1966; 14(6):892-8. PMC: 1058439. DOI: 10.1128/am.14.6.892-898.1966. View