Analysis of Recombinant Human Tumour Necrosis Factor-alpha-induced CD4 Expression on Human Eosinophils

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1996 Jun 1

PMID 8690465

Citations 2

Authors

M Hossain

Y Okubo

S Horie

M Sekiguchi

Affiliations

Soon will be listed here.

Abstract

We examined the hypothesis that one of the pro-inflammatory cytokines, tumour necrosis factor-alpha (TNF-alpha), could induce expression of the adhesion molecule CD4 on human eosinophils. We further examined the effector function of CD4 and the mechanisms regulating CD4 expression. Human eosinophils were cultured with various concentrations of recombinant human TNF-alpha (rhTNF-alpha) with or without various drugs for 24 hr. After culture, eosinophils were stained for CD4 using a monoclonal antibody and then analysed by flow cytometry. Eosinophil-derived neurotoxin (EDN) release as eosinophil degranulation was examined by cross-linking of CD4 on eosinophils. The rhTNF-alpha induced CD4 expression on human eosinophils in a dose- and time-dependent fashion; rhTNF-alpha-induced CD4 expression was significantly inhibited by 10(-6) M cycloheximide, 10(-8) M dexamethasone, or 10(-6) M herbimycin A. Recombinant human interferon-gamma inhibited rhTNF-alpha-induced CD4 expression in a dose-dependent manner. However, cross-linking of CD4 on eosinophils did not evoke EDN release, suggesting that newly expressed CD4 molecules on human eosinophils do not play any role in triggering degranulation. Our data indicate that TNF-alpha-induced CD4 expression on human eosinophils is dependent on protein synthesis and may be dependent on tyrosine kinase activity.

Citing Articles

Alloimmunization to transfused platelets requires priming of CD4+ T cells in the splenic microenvironment in a murine model.

Gilson C, Zimring J Transfusion. 2011; 52(4):849-59.

PMID: 21981241 PMC: 3257367. DOI: 10.1111/j.1537-2995.2011.03346.x.

Functionally distinct subsets of human NK cells and monocyte/DC-like cells identified by coexpression of CD56, CD7, and CD4.

Milush J, Long B, Snyder-Cappione J, Cappione 3rd A, York V, Ndhlovu L Blood. 2009; 114(23):4823-31.

PMID: 19805616 PMC: 2786291. DOI: 10.1182/blood-2009-04-216374.

References

CONRAD M . Hematologic manifestations of parasitic infections. Semin Hematol. 1971; 8(3):267-303. View

Khair O, Devalia J, Abdelaziz M, Sapsford R, Tarraf H, Davies R . Effect of Haemophilus influenzae endotoxin on the synthesis of IL-6, IL-8, TNF-alpha and expression of ICAM-1 in cultured human bronchial epithelial cells. Eur Respir J. 1994; 7(12):2109-16. DOI: 10.1183/09031936.94.07122109. View

Stewart S, Fujimoto J, Levy R . Human T lymphocytes and monocytes bear the same Leu-3(T4) antigen. J Immunol. 1986; 136(10):3773-8. View

Gay D, Maddon P, Sekaly R, Talle M, Godfrey M, Long E . Functional interaction between human T-cell protein CD4 and the major histocompatibility complex HLA-DR antigen. Nature. 1987; 328(6131):626-9. DOI: 10.1038/328626a0. View

BLUE M, Hafler D, Daley J, Levine H, Craig K, Breitmeyer J . Regulation of T cell clone function via CD4 and CD8 molecules. Anti-CD4 can mediate two distinct inhibitory activities. J Immunol. 1988; 140(2):376-83. View

Piette J, Hirai S, Yaniv M . Constitutive synthesis of activator protein 1 transcription factor after viral transformation of mouse fibroblasts. Proc Natl Acad Sci U S A. 1988; 85(10):3401-5. PMC: 280217. DOI: 10.1073/pnas.85.10.3401. View

Lucey D, Dorsky D, Nicholson-Weller A, Weller P . Human eosinophils express CD4 protein and bind human immunodeficiency virus 1 gp120. J Exp Med. 1989; 169(1):327-32. PMC: 2189179. DOI: 10.1084/jem.169.1.327. View

Veillette A, Bolen J, Bookman M . Alterations in tyrosine protein phosphorylation induced by antibody-mediated cross-linking of the CD4 receptor of T lymphocytes. Mol Cell Biol. 1989; 9(10):4441-6. PMC: 362527. DOI: 10.1128/mcb.9.10.4441-4446.1989. View

Kelley J . Cytokines of the lung. Am Rev Respir Dis. 1990; 141(3):765-88. DOI: 10.1164/ajrccm/141.3.765. View

10.

Gordon J, Galli S . Mast cells as a source of both preformed and immunologically inducible TNF-alpha/cachectin. Nature. 1990; 346(6281):274-6. DOI: 10.1038/346274a0. View

11.

Drapier J, Wietzerbin J . IFN-gamma reduces specific binding of tumor necrosis factor on murine macrophages. J Immunol. 1991; 146(4):1198-203. View

12.

Muegge K, Durum S . Cytokines and transcription factors. Cytokine. 1990; 2(1):1-8. DOI: 10.1016/1043-4666(90)90036-s. View

13.

Hartnell A, Kay A, Wardlaw A . IFN-gamma induces expression of Fc gamma RIII (CD16) on human eosinophils. J Immunol. 1992; 148(5):1471-8. View

14.

Oshea J, McVicar D, Kuhns D, Ortaldo J . A role for protein tyrosine kinase activity in natural cytotoxicity as well as antibody-dependent cellular cytotoxicity. Effects of herbimycin A. J Immunol. 1992; 148(8):2497-502. View

15.

Hansel T, de Vries I, Carballido J, Braun R, Carballido-Perrig N, Rihs S . Induction and function of eosinophil intercellular adhesion molecule-1 and HLA-DR. J Immunol. 1992; 149(6):2130-6. View

16.

Beil W, Weller P, Tzizik D, Galli S, Dvorak A . Ultrastructural immunogold localization of tumor necrosis factor-alpha to the matrix compartment of eosinophil secondary granules in patients with idiopathic hypereosinophilic syndrome. J Histochem Cytochem. 1993; 41(11):1611-5. DOI: 10.1177/41.11.8409368. View

17.

Horie S, Kita H . CD11b/CD18 (Mac-1) is required for degranulation of human eosinophils induced by human recombinant granulocyte-macrophage colony-stimulating factor and platelet-activating factor. J Immunol. 1994; 152(11):5457-67. View

18.

Zhang F, Hausen A, Hoffmann R, Grewe M, Decker K . Rat liver macrophages express the 55 kDa tumor necrosis factor receptor: modulation by interferon-gamma, lipopolysaccharide and tumor necrosis factor-alpha. Biol Chem Hoppe Seyler. 1994; 375(4):249-54. DOI: 10.1515/bchm3.1994.375.4.249. View

19.

Yang J, Merin J, Nakano T, Kato T, Kitade Y, Okamoto T . Inhibition of the DNA-binding activity of NF-kappa B by gold compounds in vitro. FEBS Lett. 1995; 361(1):89-96. DOI: 10.1016/0014-5793(95)00157-5. View

20.

Okubo Y, Hossain M, Kai R, Sato E, Honda T, Sekiguchi M . Adhesion molecules on eosinophils in acute eosinophilic pneumonia. Am J Respir Crit Care Med. 1995; 151(4):1259-62. DOI: 10.1164/ajrccm/151.4.1259. View