Functional Expression of the Extraplastidial Arabidopsis Thaliana Oleate Desaturase Gene (FAD2) in Saccharomyces Cerevisiae

Overview

Journal Plant Physiol

Specialty Physiology

Date 1996 May 1

PMID 8685264

Citations 37

Authors

P S Covello

D W Reed

Affiliations

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Abstract

The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces cerevisiae cells and were confirmed to be linoleic acid and delta 9, delta 12-hexadecadienoic acid by gas chromatography-mass spectrometry.

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