Peculiarities of the Regulation of Fermentation and Respiration in the Crabtree-negative, Xylose-fermenting Yeast Pichia Stipitis

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 1996 Jan 1

PMID 8669897

Citations 22

Authors

V Passoth

M Zimmermann

U Klinner

Affiliations

Soon will be listed here.

Abstract

The respiration of Pichia stipitis was not repressed by either high concentrations of fermentable sugars or oxygen limitation. Fermentation was not induced by high sugar concentrations, but was inactivated by aerobic conditions. The activity of pyruvate dehydrogenase was constitutive. In contrast, pyruvate decarboxylase, alcohol dehydrogenase, and aldehyde dehydrogenase were induced by a reduction in the oxygen tension. It was demonstrated that in P. stipitis, the pyruvate decarboxylase is not induced by a signal from glycolysis. Contrary to Saccharomyces cerevisiae, the pyruvate decarboxylase was not inhibited by phosphate.

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References

Ciriacy M, Breitenbach I . Physiological effects of seven different blocks in glycolysis in Saccharomyces cerevisiae. J Bacteriol. 1979; 139(1):152-60. PMC: 216840. DOI: 10.1128/jb.139.1.152-160.1979. View

Polakis E, Bartley W . Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources. Biochem J. 1965; 97(1):284-97. PMC: 1264573. DOI: 10.1042/bj0970284. View

Schmitt H, ZIMMERMANN F . Genetic analysis of the pyruvate decarboxylase reaction in yeast glycolysis. J Bacteriol. 1982; 151(3):1146-52. PMC: 220390. DOI: 10.1128/jb.151.3.1146-1152.1982. View

Kurtzman C . Candida shehatae--genetic diversity and phylogenetic relationships with other xylose-fermenting yeasts. Antonie Van Leeuwenhoek. 1990; 57(4):215-22. DOI: 10.1007/BF00400153. View

Pateman J, Doy C, Olsen J, Norris U, CREASER E, Hynes M . Regulation of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (AldDH) in Aspergillus nidulans. Proc R Soc Lond B Biol Sci. 1983; 217(1208):243-64. DOI: 10.1098/rspb.1983.0009. View

Steinman C, Jakoby W . Yeast aldehyde dehydrogenase. II. Properties of the homogeneous enzyme preparations. J Biol Chem. 1968; 243(4):730-4. View

Kresze G, Ronft H . Pyruvate dehydrogenase complex from baker's yeast. 1. Purification and some kinetic and regulatory properties. Eur J Biochem. 1981; 119(3):573-9. DOI: 10.1111/j.1432-1033.1981.tb05646.x. View

Rizzi M, Klein C, Schulze C, Bui-Thanh N, Dellweg H . Xylose fermentation by yeasts. 5. Use of ATP balances for modeling oxygen-limited growth and fermentation of yeast Pichia stipitis with xylose as carbon source. Biotechnol Bioeng. 1989; 34(4):509-14. DOI: 10.1002/bit.260340411. View

Polakis E, Bartley W, Meek G . Changes in the structure and enzyme activity of Saccharomyces cerevisiae in response to changes in the environment. Biochem J. 1964; 90(2):369-74. PMC: 1202626. DOI: 10.1042/bj0900369. View

10.

Maitra P, Lobo Z . A kinetic study of glycolytic enzyme synthesis in yeast. J Biol Chem. 1971; 246(2):475-88. View

11.

Sierkstra L, Nouwen N, Verbakel J, Verrips C . Regulation of glycolytic enzymes and the Crabtree effect in galactose-limited continuous cultures of Saccharomyces cerevisiae. Yeast. 1993; 9(7):787-95. DOI: 10.1002/yea.320090713. View

12.

Keha E, Ronft H, Kresze G . On the origin of mitochondria: a reexamination of the molecular structure and kinetic properties of pyruvate dehydrogenase complex from brewer's yeast. FEBS Lett. 1982; 145(2):289-92. DOI: 10.1016/0014-5793(82)80185-3. View

13.

Hess B . A computer program for the determination of kinetic parameters from sigmoidal steady-state kinetics. FEBS Lett. 1970; 8(4):178-185. DOI: 10.1016/0014-5793(70)80258-7. View

14.

Skoog K, Hahn-Hagerdal B . Effect of Oxygenation on Xylose Fermentation by Pichia stipitis. Appl Environ Microbiol. 1990; 56(11):3389-94. PMC: 184958. DOI: 10.1128/aem.56.11.3389-3394.1990. View

15.

Boiteux A, Hess B . Allosteric properties of yeast pyruvate decarboxylase. FEBS Lett. 1970; 9(5):293-296. DOI: 10.1016/0014-5793(70)80381-7. View

16.

Boles E, Heinisch J, ZIMMERMANN F . Different signals control the activation of glycolysis in the yeast Saccharomyces cerevisiae. Yeast. 1993; 9(7):761-70. DOI: 10.1002/yea.320090710. View

17.

Ullrich J, Donner I . Kinetic evidence for two active sites in cytoplasmic yeast pyruvate decarboxylase. Hoppe Seylers Z Physiol Chem. 1970; 351(8):1026-9. DOI: 10.1515/bchm2.1970.351.2.1026. View

18.

Bradbury S, Clark J, Steinman C, Jakoby W . Aldehyde dehydrogenase from baker's yeast. Methods Enzymol. 1975; 41:354-60. DOI: 10.1016/s0076-6879(75)41079-5. View