Molecule by Molecule PCR Amplification of Complex DNA Mixtures for Direct Sequencing: an Approach to in Vitro Cloning

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1996 Jun 1

PMID 8668555

Citations 9

Authors

K A Lukyanov

M V Matz

E A Bogdanova

N G Gurskaya

S A Lukyanov

Affiliations

Soon will be listed here.

Citing Articles

Artifactual mutations resulting from DNA lesions limit detection levels in ultrasensitive sequencing applications.

Arbeithuber B, Makova K, Tiemann-Boege I DNA Res. 2016; 23(6):547-559.

PMID: 27477585 PMC: 5144678. DOI: 10.1093/dnares/dsw038.

Subtractive and differential hybridization molecular analyses of Ceratitis capitata XX/XY versus XX embryos to search for male-specific early transcribed genes.

Salvemini M, DAmato R, Petrella V, Ippolito D, Ventre G, Zhang Y BMC Genet. 2014; 15 Suppl 2:S5.

PMID: 25472628 PMC: 4255797. DOI: 10.1186/1471-2156-15-S2-S5.

No control genes required: Bayesian analysis of qRT-PCR data.

Matz M, Wright R, Scott J PLoS One. 2013; 8(8):e71448.

PMID: 23977043 PMC: 3747227. DOI: 10.1371/journal.pone.0071448.

Live imaging of multicolor-labeled cells in Drosophila.

Boulina M, Samarajeewa H, Baker J, Kim M, Chiba A Development. 2013; 140(7):1605-13.

PMID: 23482495 PMC: 3596998. DOI: 10.1242/dev.088930.

A novel and simple PCR walking method for rapid acquisition of long DNA sequence flanking a known site in microbial genome.

Luo P, Su T, Hu C, Ren C Mol Biotechnol. 2010; 47(3):220-8.

PMID: 20878504 DOI: 10.1007/s12033-010-9332-z.

References

Siebert P, Chenchik A, Kellogg D, Lukyanov K, Lukyanov S . An improved PCR method for walking in uncloned genomic DNA. Nucleic Acids Res. 1995; 23(6):1087-8. PMC: 306810. DOI: 10.1093/nar/23.6.1087. View

Lukianov S, Gurskaia N, Lukianov K, Tarabykin V, Sverdlov E . [Highly-effective subtractive hybridization of cDNA]. Bioorg Khim. 1994; 20(6):701-4. View

Sarkar G, Bolander M . Semi exponential cycle sequencing. Nucleic Acids Res. 1995; 23(7):1269-70. PMC: 306841. DOI: 10.1093/nar/23.7.1269. View

Trower M, Burt D, Purvis I, Dykes C, Christodoulou C . Fluorescent dye-primer cycle sequencing using unpurified PCR products as templates; development of a protocol amenable to high-throughput DNA sequencing. Nucleic Acids Res. 1995; 23(12):2348-9. PMC: 307032. DOI: 10.1093/nar/23.12.2348. View