Differences Between MyoD DNA Binding and Activation Site Requirements Revealed by Functional Random Sequence Selection

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1996 Jul 1

PMID 8668207

Citations 15

Authors

J Huang

T K Blackwell

L Kedes

H Weintraub

Affiliations

Soon will be listed here.

Abstract

A method has been developed for selecting functional enhancer/promoter sites from random DNA sequences in higher eukaryotic cells. Of sequences that were thus selected for transcriptional activation by the muscle-specific basic helix-loop-helix protein MyoD, only a subset are similar to the preferred in vitro binding consensus, and in the same promoter context an optimal in vitro binding site was inactive. Other sequences with full transcriptional activity instead exhibit sequence preferences that, remarkably, are generally either identical or very similar to those found in naturally occurring muscle-specific promoters. This first systematic examination of the relation between DNA binding and transcriptional activation by basic helix-loop-helix proteins indicates that binding per se is necessary but not sufficient for transcriptional activation by MyoD and implies a requirement for other DNA sequence-dependent interactions or conformations at its binding site.

Citing Articles

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References

Blackwell T, Weintraub H . Differences and similarities in DNA-binding preferences of MyoD and E2A protein complexes revealed by binding site selection. Science. 1990; 250(4984):1104-10. DOI: 10.1126/science.2174572. View

Hu J, OShea E, Kim P, Sauer R . Sequence requirements for coiled-coils: analysis with lambda repressor-GCN4 leucine zipper fusions. Science. 1990; 250(4986):1400-3. DOI: 10.1126/science.2147779. View

Lin H, Yutzey K, Konieczny S . Muscle-specific expression of the troponin I gene requires interactions between helix-loop-helix muscle regulatory factors and ubiquitous transcription factors. Mol Cell Biol. 1991; 11(1):267-80. PMC: 359617. DOI: 10.1128/mcb.11.1.267-280.1991. View

Sun X, Baltimore D . An inhibitory domain of E12 transcription factor prevents DNA binding in E12 homodimers but not in E12 heterodimers. Cell. 1991; 64(2):459-70. DOI: 10.1016/0092-8674(91)90653-g. View

Wentworth B, Donoghue M, Engert J, BERGLUND E, Rosenthal N . Paired MyoD-binding sites regulate myosin light chain gene expression. Proc Natl Acad Sci U S A. 1991; 88(4):1242-6. PMC: 50993. DOI: 10.1073/pnas.88.4.1242. View

Brennan T, Chakraborty T, Olson E . Mutagenesis of the myogenin basic region identifies an ancient protein motif critical for activation of myogenesis. Proc Natl Acad Sci U S A. 1991; 88(13):5675-9. PMC: 51940. DOI: 10.1073/pnas.88.13.5675. View

Emerson C . Myogenesis and developmental control genes. Curr Opin Cell Biol. 1990; 2(6):1065-75. DOI: 10.1016/0955-0674(90)90157-a. View

Fiering S, Roederer M, Nolan G, Micklem D, Parks D, Herzenberg L . Improved FACS-Gal: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs. Cytometry. 1991; 12(4):291-301. DOI: 10.1002/cyto.990120402. View

Wright W, Binder M, Funk W . Cyclic amplification and selection of targets (CASTing) for the myogenin consensus binding site. Mol Cell Biol. 1991; 11(8):4104-10. PMC: 361222. DOI: 10.1128/mcb.11.8.4104-4110.1991. View

10.

Lassar A, Davis R, Wright W, Kadesch T, Murre C, Voronova A . Functional activity of myogenic HLH proteins requires hetero-oligomerization with E12/E47-like proteins in vivo. Cell. 1991; 66(2):305-15. DOI: 10.1016/0092-8674(91)90620-e. View

11.

Weintraub H, Dwarki V, Verma I, Davis R, Hollenberg S, Snider L . Muscle-specific transcriptional activation by MyoD. Genes Dev. 1991; 5(8):1377-86. DOI: 10.1101/gad.5.8.1377. View

12.

Gilmour B, Fanger G, Newton C, Evans S, Gardner P . Multiple binding sites for myogenic regulatory factors are required for expression of the acetylcholine receptor gamma-subunit gene. J Biol Chem. 1991; 266(30):19871-4. View

13.

Hanahan D, Jessee J, Bloom F . Plasmid transformation of Escherichia coli and other bacteria. Methods Enzymol. 1991; 204:63-113. DOI: 10.1016/0076-6879(91)04006-a. View

14.

Aurade F, Pinset C, Chafey P, Gros F, Montarras D . Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype. Differentiation. 1994; 55(3):185-92. DOI: 10.1046/j.1432-0436.1994.5530185.x. View

15.

Zingg J, Pedraza-Alva G, Jost J . MyoD1 promoter autoregulation is mediated by two proximal E-boxes. Nucleic Acids Res. 1994; 22(12):2234-41. PMC: 523679. DOI: 10.1093/nar/22.12.2234. View

16.

Li H, Capetanaki Y . An E box in the desmin promoter cooperates with the E box and MEF-2 sites of a distal enhancer to direct muscle-specific transcription. EMBO J. 1994; 13(15):3580-9. PMC: 395263. DOI: 10.1002/j.1460-2075.1994.tb06665.x. View

17.

Genetta T, Ruezinsky D, Kadesch T . Displacement of an E-box-binding repressor by basic helix-loop-helix proteins: implications for B-cell specificity of the immunoglobulin heavy-chain enhancer. Mol Cell Biol. 1994; 14(9):6153-63. PMC: 359142. DOI: 10.1128/mcb.14.9.6153-6163.1994. View

18.

Ellenberger T, Fass D, Arnaud M, Harrison S . Crystal structure of transcription factor E47: E-box recognition by a basic region helix-loop-helix dimer. Genes Dev. 1994; 8(8):970-80. DOI: 10.1101/gad.8.8.970. View

19.

Weintraub H, Genetta T, Kadesch T . Tissue-specific gene activation by MyoD: determination of specificity by cis-acting repression elements. Genes Dev. 1994; 8(18):2203-11. DOI: 10.1101/gad.8.18.2203. View

20.

Benezra R . An intermolecular disulfide bond stabilizes E2A homodimers and is required for DNA binding at physiological temperatures. Cell. 1994; 79(6):1057-67. DOI: 10.1016/0092-8674(94)90036-1. View