Salt-induced Structural Changes in Nucleosomes

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1977 Apr 1

PMID 866195

Citations 9

Authors

C G Sahasrabuddhe

G F Saunders

Affiliations

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Abstract

Nucleosomes and oligonucleosomes were prepared by digestion of human placental nuclei with staphlococcal nuclease and fractionated by gel filtration chromatography. The effect of increasing salt on the structure of nucleosomes was examined in the presence and absence of 10 mM MgCl2. Nucleosomes and oligonucleosomes are insoluble over a broad range of salt concentration. Nucleosomes are insoluble in larger than or equal to 120 mM (NH4)2SO4 containing 10 mM MgCl2 allowing analyses of changes in nucleosomal DNA by C.D. spectroscopy. Nucleosomes are insoluble in less than or equal to 120 mM (NH4)2SO4 containing 10 mM MgCl2 as demonstrated by turbidity measurements. We conclude that the insolubility of nucleosomes accompanies salt-induced structural changes possibly due to individual particle condensation. As the salt concentration is increased the nucleosomes condense and then relax at higher salt concentrations.

Citing Articles

Chromosomal localization of the human placental lactogen-growth hormone gene cluster to 17q22-24.

Harper M, Barrera-Saldana H, Saunders G Am J Hum Genet. 1982; 34(2):227-34.

PMID: 7072716 PMC: 1685277.

Use of RNA polymerase as an enzymatic probe of nucleosomal structure.

Hodo 3rd H, Sahasrabuddhe C, Plishker M, Saunders G Nucleic Acids Res. 1980; 8(17):3851-64.

PMID: 7003538 PMC: 324199. DOI: 10.1093/nar/8.17.3851.

Chromosome-bound mitotic factors: release by endonucleases.

Adlakha R, Sahasrabuddhe C, Wright D, LINDSEY W, Smith M, Rao P Nucleic Acids Res. 1982; 10(13):4107-17.

PMID: 6287433 PMC: 320782. DOI: 10.1093/nar/10.13.4107.

Responses of mammalian metaphase chromosomes to endonuclease digestion.

Sahasrabuddhe C, Pathak S, Hsu T Chromosoma. 1978; 69(3):331-8.

PMID: 743903 DOI: 10.1007/BF00332136.

Transcription of nucleosomes from human chromatin.

Shaw P, Sahasrabuddhe C, Hodo 3rd H, Saunders G Nucleic Acids Res. 1978; 5(8):2999-3012.

PMID: 693325 PMC: 342222. DOI: 10.1093/nar/5.8.2999.

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