A Cleavage-site-directed Inhibitor of Interleukin-1 Beta-converting Enzyme-like Proteases Inhibits Apoptosis in Primary Cultures of Rat Hepatocytes

Overview

Journal Biochem J

Specialty Biochemistry

Date 1996 Feb 15

PMID 8660294

Citations 16

Authors

K Cain

S H Inayat-Hussain

C Couet

G M Cohen

Affiliations

Soon will be listed here.

Abstract

Apoptosis induced in primary hepatocytes by transforming growth factor beta1 and staurosporine produced chromatin condensation, DNA cleavage is detected by in situ end-labelling, field inversion and conventional gel electrophoresis, and cell detachment. These effects were abolished by benzyloxycarbonyl-valinylalanylaspartylfluoromethyl ketone, a cleavage-site-directed inhibitor of interleukin-1beta-converting enzyme-like proteases, and this finding suggests that these enzymes are involved in liver apoptosis.

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References

Cain K, Inayat-Hussain S, Wolfe J, Cohen G . DNA fragmentation into 200-250 and/or 30-50 kilobase pair fragments in rat liver nuclei is stimulated by Mg2+ alone and Ca2+/Mg2+ but not by Ca2+ alone. FEBS Lett. 1994; 349(3):385-91. DOI: 10.1016/0014-5793(94)80001-4. View

Weaver V, Lach B, Walker P, Sikorska M . Role of proteolysis in apoptosis: involvement of serine proteases in internucleosomal DNA fragmentation in immature thymocytes. Biochem Cell Biol. 1993; 71(9-10):488-500. DOI: 10.1139/o93-071. View

Lazebnik Y, Kaufmann S, Desnoyers S, Poirier G, Earnshaw W . Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature. 1994; 371(6495):346-7. DOI: 10.1038/371346a0. View

Ni R, Tomita Y, Matsuda K, Ichihara A, Ishimura K, Ogasawara J . Fas-mediated apoptosis in primary cultured mouse hepatocytes. Exp Cell Res. 1994; 215(2):332-7. DOI: 10.1006/excr.1994.1349. View

Fernandes-Alnemri T, Litwack G, Alnemri E . CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J Biol Chem. 1994; 269(49):30761-4. View

Miller B, Krasney P, Gauvin D, Holbrook K, Koonz D, Abruzzese R . Inhibition of mature IL-1 beta production in murine macrophages and a murine model of inflammation by WIN 67694, an inhibitor of IL-1 beta converting enzyme. J Immunol. 1995; 154(3):1331-8. View

Fearnhead H, Rivett A, Dinsdale D, Cohen G . A pre-existing protease is a common effector of thymocyte apoptosis mediated by diverse stimuli. FEBS Lett. 1995; 357(3):242-6. DOI: 10.1016/0014-5793(94)01367-a. View

Derynck R . TGF-beta-receptor-mediated signaling. Trends Biochem Sci. 1994; 19(12):548-53. DOI: 10.1016/0968-0004(94)90059-0. View

Skilleter D, Cain K . Effects of thiol agents on the accumulation of cadmium by rat liver parenchymal and Kupffer cells. Chem Biol Interact. 1981; 37(3):289-98. DOI: 10.1016/0009-2797(81)90115-0. View

10.

McConkey D, Hartzell P, Nicotera P, Wyllie A, Orrenius S . Stimulation of endogenous endonuclease activity in hepatocytes exposed to oxidative stress. Toxicol Lett. 1988; 42(2):123-30. DOI: 10.1016/0378-4274(88)90069-0. View

11.

Shinagawa T, Yoshioka K, Kakumu S, Wakita T, Ishikawa T, Itoh Y . Apoptosis in cultured rat hepatocytes: the effects of tumour necrosis factor alpha and interferon gamma. J Pathol. 1991; 165(3):247-53. DOI: 10.1002/path.1711650309. View

12.

Ellis R, Yuan J, Horvitz H . Mechanisms and functions of cell death. Annu Rev Cell Biol. 1991; 7:663-98. DOI: 10.1146/annurev.cb.07.110191.003311. View

13.

Oberhammer F, Pavelka M, Sharma S, Tiefenbacher R, Purchio A, Bursch W . Induction of apoptosis in cultured hepatocytes and in regressing liver by transforming growth factor beta 1. Proc Natl Acad Sci U S A. 1992; 89(12):5408-12. PMC: 49301. DOI: 10.1073/pnas.89.12.5408. View

14.

Kumar S, Tomooka Y, Noda M . Identification of a set of genes with developmentally down-regulated expression in the mouse brain. Biochem Biophys Res Commun. 1992; 185(3):1155-61. DOI: 10.1016/0006-291x(92)91747-e. View

15.

Mackintosh C, MacKintosh R . Inhibitors of protein kinases and phosphatases. Trends Biochem Sci. 1994; 19(11):444-8. DOI: 10.1016/0968-0004(94)90127-9. View

16.

Li P, Allen H, Banerjee S, Franklin S, Herzog L, Johnston C . Mice deficient in IL-1 beta-converting enzyme are defective in production of mature IL-1 beta and resistant to endotoxic shock. Cell. 1995; 80(3):401-11. DOI: 10.1016/0092-8674(95)90490-5. View

17.

Los M, van de Craen M, Penning L, Schenk H, Westendorp M, Baeuerle P . Requirement of an ICE/CED-3 protease for Fas/APO-1-mediated apoptosis. Nature. 1995; 375(6526):81-3. DOI: 10.1038/375081a0. View

18.

Mashima T, Naito M, Kataoka S, Kawai H, Tsuruo T . Aspartate-based inhibitor of interleukin-1 beta-converting enzyme prevents antitumor agent-induced apoptosis in human myeloid leukemia U937 cells. Biochem Biophys Res Commun. 1995; 209(3):907-15. DOI: 10.1006/bbrc.1995.1584. View

19.

Faucheu C, Diu A, Chan A, Blanchet A, Miossec C, Herve F . A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells. EMBO J. 1995; 14(9):1914-22. PMC: 398290. DOI: 10.1002/j.1460-2075.1995.tb07183.x. View

20.

Chow S, Weis M, Kass G, Holmstrom T, Eriksson J, Orrenius S . Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes. FEBS Lett. 1995; 364(2):134-8. DOI: 10.1016/0014-5793(95)00370-o. View