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Immunolocalization of an Inwardly Rectifying K+ Channel, K(AB)-2 (Kir4.1), in the Basolateral Membrane of Renal Distal Tubular Epithelia

Overview
Journal FEBS Lett
Specialty Biochemistry
Date 1996 Jun 10
PMID 8654579
Citations 46
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Abstract

Immunolocalization of K(AB)-2 (Kir4.1), an inwardly rectifying K+ channel with a putative ATP-binding domain, was examined in rat kidney where expression of K(AB)-2 mRNA was previously shown. Anti-K(AB)-2 antibody was raised in rabbit and then affinity-purified. An immunohistochemical study revealed that K(AB)-2 immunoreactivity was detected specifically in the basolateral membrane of distal tubular epithelia. Therefore, K(AB)-2 is the first K+ channel shown to be localized in the basolateral membrane of renal epithelia. The finding suggests that K(AB)-2 may contribute to supplying K+ to the Na(+)-K+ pump, which is abundant in the basolateral membrane of distal tubular epithelia, as well as to maintenance of the deep negative membrane potential of these cells.

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