A New Set of Versatile Vectors for the Heterologous Expression of Foreign Genes Using the Baculovirus System
Overview
Affiliations
A new set of versatile advanced baculovirus (BV) vectors for the production of fused proteins in insect cells, under the control of the strong polyhedrin promoter of the Autographa california nuclear polyhedrosis virus (AcMNPV), has been constructed. The vectors contain peptide tags which allow immunological detection, as well as purification of recombinant protein produced via the BV expression system.
Peptides from the SARS-associated coronavirus as tags for protein expression and purification.
Gruschke S, Bussmann B, Reiche S, Jassoy C Protein Expr Purif. 2008; 61(2):138-41.
PMID: 18565762 PMC: 7129034. DOI: 10.1016/j.pep.2008.04.011.
Marheineke K, Lenhard T, Haase W, Beckers T, Michel H, Reilander H Cell Mol Neurobiol. 1998; 18(5):509-24.
PMID: 9777251 PMC: 11560213. DOI: 10.1023/a:1026379326184.