Evaluation of the Thymidine Kinase (tk) Locus As an Insertion Site in the Highly Attenuated Vaccinia MVA Strain

Overview

Journal Arch Virol

Specialty Microbiology

Date 1996 Jan 1

PMID 8645102

Citations 6

Authors

F Scheiflinger

F G Falkner

F Dorner

Affiliations

Soon will be listed here.

Abstract

The highly attenuated 'modified vaccinia Ankara' (MVA) strain is a potential live vaccine vector. Insertional inactivation of the tk-gene resulted in viruses difficult to purify. Co-integration of a functional fowlpox virus tk-gene allowed easy generation of recombinants, indicating that the genetically stable tk-gene region is a suitable insertion site, if tk-gene activity is substituted.

Citing Articles

Transgene expression knock-down in recombinant Modified Vaccinia virus Ankara vectors improves genetic stability and sustained transgene maintenance across multiple passages.

Neckermann P, Mohr M, Billmeier M, Karlas A, Boilesen D, Thirion C Front Immunol. 2024; 15:1338492.

PMID: 38380318 PMC: 10877035. DOI: 10.3389/fimmu.2024.1338492.

A T cell-targeted multi-antigen vaccine generates robust cellular and humoral immunity against SARS-CoV-2 infection.

Boulton S, Poutou J, Gill R, Alluqmani N, He X, Singaravelu R Mol Ther Methods Clin Dev. 2023; 31:101110.

PMID: 37822719 PMC: 10562195. DOI: 10.1016/j.omtm.2023.101110.

Generation of Recombinant Vaccinia Viruses.

Wyatt L, Earl P, Moss B Curr Protoc Protein Sci. 2017; 89:5.13.1-5.13.18.

PMID: 28762491 PMC: 5765993. DOI: 10.1002/cpps.33.

Generation of Recombinant Vaccinia Viruses.

Wyatt L, Earl P, Moss B Curr Protoc Microbiol. 2015; 39:14A.4.1-14A.4.18.

PMID: 26528782 PMC: 5123791. DOI: 10.1002/9780471729259.mc14a04s39.

SMAC-armed vaccinia virus induces both apoptosis and necroptosis and synergizes the efficiency of vinblastine in HCC.

Pan Q, Huang Y, Chen L, Gu J, Zhou X Hum Cell. 2014; 27(4):162-71.

PMID: 24771354 DOI: 10.1007/s13577-014-0093-z.

References

STICKL H, Hochstein-Mintzel V, Mayr A, Huber H, Schafer H, Holzner A . [MVA vaccination against smallpox: clinical tests with an attenuated live vaccinia virus strain (MVA) (author's transl)]. Dtsch Med Wochenschr. 1974; 99(47):2386-92. DOI: 10.1055/s-0028-1108143. View

Mackett M, Smith G, Moss B . Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc Natl Acad Sci U S A. 1982; 79(23):7415-9. PMC: 347350. DOI: 10.1073/pnas.79.23.7415. View

Altenburger W, Suter C, Altenburger J . Partial deletion of the human host range gene in the attenuated vaccinia virus MVA. Arch Virol. 1989; 105(1-2):15-27. DOI: 10.1007/BF01311113. View

Buller R, Smith G, Cremer K, Notkins A, Moss B . Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype. Nature. 1985; 317(6040):813-5. DOI: 10.1038/317813a0. View

Sutter G, Wyatt L, Foley P, Bennink J, Moss B . A recombinant vector derived from the host range-restricted and highly attenuated MVA strain of vaccinia virus stimulates protective immunity in mice to influenza virus. Vaccine. 1994; 12(11):1032-40. DOI: 10.1016/0264-410x(94)90341-7. View

Moss B . Vaccinia virus: a tool for research and vaccine development. Science. 1991; 252(5013):1662-7. DOI: 10.1126/science.2047875. View

Sugimoto M, Yamanouchi K . Characteristics of an attenuated vaccinia virus strain, LC16m0, and its recombinant virus vaccines. Vaccine. 1994; 12(8):675-81. DOI: 10.1016/0264-410x(94)90215-1. View

Spehner D, Drillien R, Lecocq J . Construction of fowlpox virus vectors with intergenic insertions: expression of the beta-galactosidase gene and the measles virus fusion gene. J Virol. 1990; 64(2):527-33. PMC: 249140. DOI: 10.1128/JVI.64.2.527-533.1990. View

KEMPE C, FULGINITI V, Minamitani M, Shinefield H . Smallpox vaccination of eczema patients with a strain of attenuated live vaccinia (CVI-78). Pediatrics. 1968; 42(6):980-5. View

10.

Meyer H, Sutter G, Mayr A . Mapping of deletions in the genome of the highly attenuated vaccinia virus MVA and their influence on virulence. J Gen Virol. 1991; 72 ( Pt 5):1031-8. DOI: 10.1099/0022-1317-72-5-1031. View

11.

Chakrabarti S, Brechling K, Moss B . Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques. Mol Cell Biol. 1985; 5(12):3403-9. PMC: 369169. DOI: 10.1128/mcb.5.12.3403-3409.1985. View

12.

Tartaglia J, Perkus M, Taylor J, Norton E, Audonnet J, Cox W . NYVAC: a highly attenuated strain of vaccinia virus. Virology. 1992; 188(1):217-32. DOI: 10.1016/0042-6822(92)90752-b. View

13.

Mayr A, STICKL H, Muller H, Danner K, Singer H . [The smallpox vaccination strain MVA: marker, genetic structure, experience gained with the parenteral vaccination and behavior in organisms with a debilitated defence mechanism (author's transl)]. Zentralbl Bakteriol B. 1978; 167(5-6):375-90. View

14.

Sutter G, Moss B . Nonreplicating vaccinia vector efficiently expresses recombinant genes. Proc Natl Acad Sci U S A. 1992; 89(22):10847-51. PMC: 50439. DOI: 10.1073/pnas.89.22.10847. View

15.

Redfield R, Wright D, James W, Jones T, Brown C, Burke D . Disseminated vaccinia in a military recruit with human immunodeficiency virus (HIV) disease. N Engl J Med. 1987; 316(11):673-6. DOI: 10.1056/NEJM198703123161106. View

16.

Falkner F, Moss B . Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors. J Virol. 1988; 62(6):1849-54. PMC: 253265. DOI: 10.1128/JVI.62.6.1849-1854.1988. View

17.

Boyle D, Coupar B, GIBBS A, Seigman L, Both G . Fowlpox virus thymidine kinase: nucleotide sequence and relationships to other thymidine kinases. Virology. 1987; 156(2):355-65. DOI: 10.1016/0042-6822(87)90415-6. View