The T(10;11)(p13;q14) in the U937 Cell Line Results in the Fusion of the AF10 Gene and CALM, Encoding a New Member of the AP-3 Clathrin Assembly Protein Family

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1996 May 14

PMID 8643484

Citations 109

Authors

M H Dreyling

J A Martinez-Climent

M Zheng

J Mao

J D Rowley

S K Bohlander

Affiliations

Soon will be listed here.

Abstract

The translocation t(10;11)(p13;q14) is a recurring chromosomal abnormality that has been observed in patients with acute lymphoblastic leukemia as well as acute myeloid leukemia. We have recently reported that the monocytic cell line U937 has a t(10;11)(p13;q14) translocation. Using a combination of positional cloning and candidate gene approach, we cloned the breakpoint and were able to show that AF10 is fused to a novel gene that we named CALM (Clathrin Assembly Lymphoid Myeloid leukemia gene) located at 11q14. AF10, a putative transcription factor, had recently been cloned as one of the fusion partners of MLL. CALM has a very high homology in its N-terminal third to the murine ap-3 gene which is one of the clathrin assembly proteins. The N-terminal region of ap-3 has been shown to bind to clathrin and to have a high-affinity binding site for phosphoinositols. The identification of the CALM/AF10 fusion gene in the widely used U937 cell line will contribute to our understanding of the malignant phenotype of this line.

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