Rapid and Sensitive Polymerase Chain Reaction Based Detection and Typing of Foot-and-mouth Disease Virus in Clinical Samples and Cell Culture Isolates, Combined with a Simultaneous Differentiation with Other Genomically And/or Symptomatically...

Overview

Journal Arch Virol

Specialty Microbiology

Date 1996 Jan 1

PMID 8634024

Citations 26

Authors

W Vangrysperre

K De Clercq

Affiliations

Soon will be listed here.

Abstract

Reverse transcription followed by the polymerase chain reaction method (PCR) allowed the detection of foot-and-mouth disease virus (FMDV), regardless of the serotype. A primer set corresponding to highly conserved regions of the 2B sequence was selected. By combining in a single reaction tube specific primer pairs for FMDV, swine vesicular disease virus, (SVDV), encephalomyocarditis virus (EMCV) and bovine viral diarrhea virus (BVDV), all four viruses could be identified and differentiated in one amplification reaction, based on the different lengths of the respective amplified segments. In a similar way, FMDV types O, A, C, SAT 2 and Asia 1 could be identified and differentiated, using primers selected from the ID (VP1) genome region. All results were confirmed by direct sequencing of the PCR product. The very fast RNA extraction, reverse transcription and PCR permitted us to read the agarose gels within three hours after samples (cell culture isolates as well as clinical material) arrived, which is of great importance in case of an FMDV suspicion. Furthermore, a very high sensitivity was achieved (less than one PFU). Therefore, our powerful detection assay by means of PCR for FMDV as well as for SVDV, EMCV and BVDV, has advantages compared to the presently used procedures.

Citing Articles

IFN-stimulated metabolite transporter ENT3 facilitates viral genome release.

Hsieh Y, Tsai T, Huang S, Heng J, Huang Y, Tsai P EMBO Rep. 2023; 24(3):e55286.

PMID: 36652307 PMC: 9986816. DOI: 10.15252/embr.202255286.

Development of reverse-transcriptase, real-time PCR assays to distinguish the Southern African Territories (SAT) serotypes 1 and 3 and topotype VII of SAT2 of Foot-and-Mouth Disease Virus.

Chestley T, Sroga P, Nebroski M, Hole K, Ularamu H, Lung O Front Vet Sci. 2022; 9:977761.

PMID: 36204292 PMC: 9530708. DOI: 10.3389/fvets.2022.977761.

Enhanced detection and serotyping of foot-and-mouth disease virus serotype O, A, and Asia1 using a novel multiplex real-time RT-PCR.

Lim D, Ryoo S, Kang H, Oh S, Jang S, Kang B Transbound Emerg Dis. 2022; 69(5):e2578-e2589.

PMID: 35614493 PMC: 9796456. DOI: 10.1111/tbed.14603.

Detection of an emerging novel sublineage Ind2001BD1 and lineage PanAsia of foot-and-mouth disease virus serotype O in cattle in Manikgonj district of Bangladesh, 2018.

Ali M, Giasuddin M Open Vet J. 2020; 10(3):347-353.

PMID: 33282707 PMC: 7703609. DOI: 10.4314/ovj.v10i3.14.

Genetic Basis of Antigenic Variation of SAT3 Foot-And-Mouth Disease Viruses in Southern Africa.

Maake L, Harvey W, Rotherham L, Opperman P, Theron J, Reeve R Front Vet Sci. 2020; 7:568.

PMID: 33102544 PMC: 7506032. DOI: 10.3389/fvets.2020.00568.

References

Palmenberg A, Kirby E, Janda M, DRAKE N, Duke G, Potratz K . The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region. Nucleic Acids Res. 1984; 12(6):2969-85. PMC: 318719. DOI: 10.1093/nar/12.6.2969. View

Hertig C, Pauli U, Zanoni R, Peterhans E . Detection of bovine viral diarrhea (BVD) virus using the polymerase chain reaction. Vet Microbiol. 1991; 26(1-2):65-76. DOI: 10.1016/0378-1135(91)90042-e. View

Rodriguez A, Nunez J, Nolasco G, Ponz F, Sobrino F, de Blas C . Direct PCR detection of foot-and-mouth disease virus. J Virol Methods. 1994; 47(3):345-9. DOI: 10.1016/0166-0934(94)90030-2. View

Laor O, Torgersen H, Yadin H, Becker Y . Detection of FMDV RNA amplified by the polymerase chain reaction (PCR). J Virol Methods. 1992; 36(3):197-207. DOI: 10.1016/0166-0934(92)90051-e. View

Colett M, Larson R, Gold C, Strick D, Anderson D, Purchio A . Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology. 1988; 165(1):191-9. DOI: 10.1016/0042-6822(88)90672-1. View

Hofner M, Carpenter W, Donaldson A . Detection of foot-and-mouth disease virus RNA in clinical samples and cell culture isolates by amplification of the capsid coding region. J Virol Methods. 1993; 42(1):53-61. DOI: 10.1016/0166-0934(93)90176-r. View

Amaral-Doel C, Owen N, Ferris N, Kitching R, Doel T . Detection of foot-and-mouth disease viral sequences in clinical specimens and ethyleneimine-inactivated preparations by the polymerase chain reaction. Vaccine. 1993; 11(4):415-21. DOI: 10.1016/0264-410x(93)90281-2. View

Koenen F, De Clercq K, Lefebvre J, Strobbe R . Reproductive failure in sows following experimental infection with a Belgian EMCV isolate. Vet Microbiol. 1994; 39(1-2):111-6. DOI: 10.1016/0378-1135(94)90091-4. View

Meyer R, Brown C, House C, House J, Molitor T . Rapid and sensitive detection of foot-and-mouth disease virus in tissues by enzymatic RNA amplification of the polymerase gene. J Virol Methods. 1991; 34(2):161-72. DOI: 10.1016/0166-0934(91)90096-i. View

10.

Locher F, Suryanarayana V, Tratschin J . Rapid detection and characterization of foot-and-mouth disease virus by restriction enzyme and nucleotide sequence analysis of PCR products. J Clin Microbiol. 1995; 33(2):440-4. PMC: 227963. DOI: 10.1128/jcm.33.2.440-444.1995. View

11.

Seechurn P, Knowles N, McCauley J . The complete nucleotide sequence of a pathogenic swine vesicular disease virus. Virus Res. 1990; 16(3):255-74. DOI: 10.1016/0168-1702(90)90052-d. View

12.

Forss S, Strebel K, Beck E, Schaller H . Nucleotide sequence and genome organization of foot-and-mouth disease virus. Nucleic Acids Res. 1984; 12(16):6587-601. PMC: 320098. DOI: 10.1093/nar/12.16.6587. View

13.

Prato Murphy M, Meyer R, Mebus C, Schudel A, Rodriguez M . Analysis of sites of foot and mouth disease virus persistence in carrier cattle via the polymerase chain reaction. Arch Virol. 1994; 136(3-4):299-307. DOI: 10.1007/BF01321059. View

14.

Donn A, Martin L, Donaldson A . Improved detection of persistent foot-and-mouth disease infection in cattle by the polymerase chain reaction. J Virol Methods. 1994; 49(2):179-86. DOI: 10.1016/0166-0934(94)90042-6. View

15.

Brocchi E, Berlinzani A, Gamba D, De Simone F . Development of two novel monoclonal antibody-based ELISAs for the detection of antibodies and the identification of swine isotypes against swine vesicular disease virus. J Virol Methods. 1995; 52(1-2):155-67. DOI: 10.1016/0166-0934(94)00158-d. View