Identification of a Unique VP4 Serotype That is Shared by a Human Rotavirus (69M Strain) and an Equine Rotavirus (H-2 Strain)

Overview

Journal Arch Virol

Specialty Microbiology

Date 1996 Jan 1

PMID 8629943

Citations 4

Authors

B Li

Y Hoshino

M Gorziglia

Affiliations

Soon will be listed here.

Abstract

A cDNA clone representing the VP4-encoding gene of human rotavirus strain 69M(VP7 serotype 8) was constructed and inserted into a baculovirus expression vector. Baculovirus recombinants that expressed the 69 M VP4 protein in Spodoptera frugiperda (Sf9) cells were screened by immunofluorescence with hyperimmune antiserum to the 69M strain and purified by terminal dilution. The expressed VP4 was detected by Coomassie blue staining of PAGE-separated proteins. The antigenic relationships between the VP4 of the 69M strain and those of various human and other animal rotavirus strains representing ten established VP4 serotypes were examined by plaque reduction neutralization. Hyperimmune antiserum produced in guinea pigs following immunization with a lysate of Sf9 cells infected with a 69M gene 4-baculovirus recombinant neutralized the infectivity of the homologous human rotavirus 69M strain as well as heterologous equine rotavirus H-2 strain to a high titer. The anti-69M VP4 hyperimmune antiserum did not neutralize significantly other rotavirus strains of human, simian, porcine, bovine, or murine origin. It thus appears that the human rotavirus 69M strain has a distinct VP4 serotype (designated as P serotype 4) which is closely related antigenically to equine rotavirus H-2 VP4.

Citing Articles

Diversity in Indian equine rotaviruses: identification of genotype G10,P6[1] and G1 strains and a new VP7 genotype (G16) strain in specimens from diarrheic foals in India.

Gulati B, Deepa R, Singh B, Rao C J Clin Microbiol. 2006; 45(3):972-8.

PMID: 17135436 PMC: 1829150. DOI: 10.1128/JCM.01696-06.

Unexpected detection of animal VP7 genes among common rotavirus strains isolated from children in Mexico.

Laird A, Ibarra V, Ruiz-Palacios G, Guerrero M, Glass R, Gentsch J J Clin Microbiol. 2003; 41(9):4400-3.

PMID: 12958276 PMC: 193830. DOI: 10.1128/JCM.41.9.4400-4403.2003.

Genetic analysis of Group A rotaviruses: evidence for interspecies transmission of rotavirus genes.

Palombo E Virus Genes. 2002; 24(1):11-20.

PMID: 11928984 DOI: 10.1023/a:1014073618253.

Isolation of serotype G8, P6[1] bovine rotavirus from adult cattle with diarrhea.

Sato M, Nakagomi T, Tajima K, Ezura K, Akashi H, Nakagomi O J Clin Microbiol. 1997; 35(5):1266-8.

PMID: 9114422 PMC: 232744. DOI: 10.1128/jcm.35.5.1266-1268.1997.

References

Hasegawa A, Inouye S, Matsuno S, Yamaoka K, Eko R, Suharyono W . Isolation of human rotaviruses with a distinct RNA electrophoretic pattern from Indonesia. Microbiol Immunol. 1984; 28(6):719-22. DOI: 10.1111/j.1348-0421.1984.tb00726.x. View

Estes M, Cohen J . Rotavirus gene structure and function. Microbiol Rev. 1989; 53(4):410-49. PMC: 372748. DOI: 10.1128/mr.53.4.410-449.1989. View

Gouvea V, de Castro L, Timenetsky M, Greenberg H, Santos N . Rotavirus serotype G5 associated with diarrhea in Brazilian children. J Clin Microbiol. 1994; 32(5):1408-9. PMC: 263717. DOI: 10.1128/jcm.32.5.1408-1409.1994. View

Snodgrass D, Hoshino Y, Fitzgerald T, Smith M, Browning G, Gorziglia M . Identification of four VP4 serological types (P serotypes) of bovine rotavirus using viral reassortants. J Gen Virol. 1992; 73 ( Pt 9):2319-25. DOI: 10.1099/0022-1317-73-9-2319. View

Hoshino Y, Wyatt R, Greenberg H, Kalica A, Flores J, Kapikian A . Isolation and characterization of an equine rotavirus. J Clin Microbiol. 1983; 18(3):585-91. PMC: 270858. DOI: 10.1128/jcm.18.3.585-591.1983. View

Isa P, Snodgrass D . Serological and genomic characterization of equine rotavirus VP4 proteins identifies three different P serotypes. Virology. 1994; 201(2):364-72. DOI: 10.1006/viro.1994.1302. View

Matsuno S, Hasegawa A, Mukoyama A, Inouye S . A candidate for a new serotype of human rotavirus. J Virol. 1985; 54(2):623-4. PMC: 254837. DOI: 10.1128/JVI.54.2.623-624.1985. View

Hoshino Y, Kapikian A . Rotavirus antigens. Curr Top Microbiol Immunol. 1994; 185:179-227. DOI: 10.1007/978-3-642-78256-5_7. View

Offit P, Shaw R, Greenberg H . Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7. J Virol. 1986; 58(2):700-3. PMC: 252967. DOI: 10.1128/JVI.58.2.700-703.1986. View

10.

Gorziglia M, Green K, Nishikawa K, Taniguchi K, Jones R, Kapikian A . Sequence of the fourth gene of human rotaviruses recovered from asymptomatic or symptomatic infections. J Virol. 1988; 62(8):2978-84. PMC: 253736. DOI: 10.1128/JVI.62.8.2978-2984.1988. View

11.

Qian Y, Green K . Human rotavirus strain 69M has a unique VP4 as determined by amino acid sequence analysis. Virology. 1991; 182(1):407-12. DOI: 10.1016/0042-6822(91)90691-4. View

12.

Matsui S, Mackow E, Greenberg H . Molecular determinant of rotavirus neutralization and protection. Adv Virus Res. 1989; 36:181-214. DOI: 10.1016/s0065-3527(08)60585-0. View

13.

Hoshino Y, Saif L, Sereno M, Chanock R, Kapikian A . Infection immunity of piglets to either VP3 or VP7 outer capsid protein confers resistance to challenge with a virulent rotavirus bearing the corresponding antigen. J Virol. 1988; 62(3):744-8. PMC: 253627. DOI: 10.1128/JVI.62.3.744-748.1988. View

14.

Albert M, Unicomb L, Bishop R . Cultivation and characterization of human rotaviruses with "super short" RNA patterns. J Clin Microbiol. 1987; 25(1):183-5. PMC: 265857. DOI: 10.1128/jcm.25.1.183-185.1987. View

15.

Hardy M, Gorziglia M, Woode G . The outer capsid protein VP4 of equine rotavirus strain H-2 represents a unique VP4 type by amino acid sequence analysis. Virology. 1993; 193(1):492-7. DOI: 10.1006/viro.1993.1152. View

16.

Nishikawa K, Fukuhara N, Liprandi F, Green K, Kapikian A, Chanock R . VP4 protein of porcine rotavirus strain OSU expressed by a baculovirus recombinant induces neutralizing antibodies. Virology. 1989; 173(2):631-7. DOI: 10.1016/0042-6822(89)90575-8. View

17.

Sereno M, Gorziglia M . The outer capsid protein VP4 of murine rotavirus strain Eb represents a tentative new P type. Virology. 1994; 199(2):500-4. DOI: 10.1006/viro.1994.1153. View

18.

Gorziglia M, Larralde G, Kapikian A, Chanock R . Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci U S A. 1990; 87(18):7155-9. PMC: 54702. DOI: 10.1073/pnas.87.18.7155. View

19.

Gorziglia M, Hoshino Y, Buckler-White A, Blumentals I, Glass R, Flores J . Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection. Proc Natl Acad Sci U S A. 1986; 83(18):7039-43. PMC: 386648. DOI: 10.1073/pnas.83.18.7039. View

20.

Prasad B, Wang G, Clerx J, Chiu W . Three-dimensional structure of rotavirus. J Mol Biol. 1988; 199(2):269-75. DOI: 10.1016/0022-2836(88)90313-0. View