Genomic Structure of the Rice Aldolase Isozyme C-1 Gene and Its Regulation Through a Ca 2+ -mediated Protein Kinase-phosphatase Pathway

Overview

Journal Plant Mol Biol

Date 1996 Jan 1

PMID 8616263

Citations 3

Authors

H Nakamura

W Satoh

S Hidaka

Y Kagaya

S Ejiri

K Tsutsumi

Affiliations

Soon will be listed here.

Abstract

Complementary and genomic DNA clones coding for aldolase C-1, the fourth-type isozyme of aldolase in rice Oryza sativa L., have been characterized. The organization of the gene is quite similar to those encoding rice aldolase C-a and a maize cytoplasmic-type aldolase, in that introns are located in the same position. Amino acid sequences are highly conserved among cytoplasmic aldolases in plants. Expression of the gene in rice callus is activated by a protein phosphatase inhibitor okadaic acid, and is inhibited in the presence of thapsigargin, a reagent which increases calcium influx into the cytoplasm. The inhibition is rescued by the simultaneous addition of protein kinase inhibitor H-7. Thus, it is suggested that expression of the aldolase C-1 gene is regulated through a signal transduction pathway involving a Ca 2+ -mediated protein kinase-protein phosphatase system.

Citing Articles

Proton export alkalinizes intracellular pH and reprograms carbon metabolism to drive normal and malignant cell growth.

Man C, Mercier F, Liu N, Dong W, Stephanopoulos G, Jiang L Blood. 2021; 139(4):502-522.

PMID: 34610101 PMC: 8796654. DOI: 10.1182/blood.2021011563.

Characterization of fructose-1,6-bisphosphate aldolase during anoxia in the tolerant turtle, Trachemys scripta elegans: an assessment of enzyme activity, expression and structure.

Dawson N, Biggar K, Storey K PLoS One. 2013; 8(7):e68830.

PMID: 23874782 PMC: 3715522. DOI: 10.1371/journal.pone.0068830.

Alteration of substrate specificity by a naturally-occurring aldolase B mutation (Ala337-->Val) in fructose intolerance.

Rellos P, Ali M, Vidailhet M, Sygusch J, Cox T Biochem J. 1999; 340 ( Pt 1):321-7.

PMID: 10229688 PMC: 1220251.

References

Ma H . Protein phosphorylation in plants: enzymes, substrates and regulators. Trends Genet. 1993; 9(7):228-30. DOI: 10.1016/0168-9525(93)90075-s. View

Anderson L, Levin D . Chloroplast aldolase is controlled by a nuclear gene. Plant Physiol. 1970; 46(6):819-20. PMC: 396689. DOI: 10.1104/pp.46.6.819. View

Hidaka S, Kadowaki K, Tsutsumi K, Ishikawa K . Nucleotide sequence of the rice cytoplasmic aldolase cDNA. Nucleic Acids Res. 1990; 18(13):3991. PMC: 331107. DOI: 10.1093/nar/18.13.3991. View

Mackintosh C, Coggins J, Cohen P . Plant protein phosphatases. Subcellular distribution, detection of protein phosphatase 2C and identification of protein phosphatase 2A as the major quinate dehydrogenase phosphatase. Biochem J. 1991; 273 ( Pt 3):733-8. PMC: 1149824. DOI: 10.1042/bj2730733. View

Sheen J . Protein phosphatase activity is required for light-inducible gene expression in maize. EMBO J. 1993; 12(9):3497-505. PMC: 413626. DOI: 10.1002/j.1460-2075.1993.tb06024.x. View

Penger A, Schnarrenberger C . Plant aldolase: cDNA and deduced amino-acid sequences of the chloroplast and cytosol enzyme from spinach. Plant Mol Biol. 1993; 21(2):331-40. DOI: 10.1007/BF00019948. View

Trewavas A, Gilroy S . Signal transduction in plant cells. Trends Genet. 1991; 7(11-12):356-61. DOI: 10.1016/0168-9525(91)90255-o. View

Kurata N, Nagamura Y, Yamamoto K, Harushima Y, Sue N, Wu J . A 300 kilobase interval genetic map of rice including 883 expressed sequences. Nat Genet. 1994; 8(4):365-72. DOI: 10.1038/ng1294-365. View

Bowler C, Neuhaus G, Yamagata H, Chua N . Cyclic GMP and calcium mediate phytochrome phototransduction. Cell. 1994; 77(1):73-81. DOI: 10.1016/0092-8674(94)90236-4. View

10.

Thastrup O, Cullen P, Drobak B, Hanley M, Dawson A . Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase. Proc Natl Acad Sci U S A. 1990; 87(7):2466-70. PMC: 53710. DOI: 10.1073/pnas.87.7.2466. View

11.

Nanmori T, Taguchi W, Kinugasa M, Oji Y, Sahara S, Fukami Y . Purification and characterization of protein kinase C from a higher plant, Brassica campestris L. Biochem Biophys Res Commun. 1994; 203(1):311-8. DOI: 10.1006/bbrc.1994.2183. View

12.

Chopra S, Dolferus R, Jacobs M . Cloning and sequencing of the Arabidopsis aldolase gene. Plant Mol Biol. 1990; 15(3):517-20. DOI: 10.1007/BF00019171. View

13.

Harper J, Sussman M, Schaller G, Putnam-Evans C, Charbonneau H, Harmon A . A calcium-dependent protein kinase with a regulatory domain similar to calmodulin. Science. 1991; 252(5008):951-4. DOI: 10.1126/science.1852075. View

14.

Tsutsumi K, Kagaya Y, Hidaka S, Suzuki J, Tokairin Y, Hirai T . Structural analysis of the chloroplastic and cytoplasmic aldolase-encoding genes implicated the occurrence of multiple loci in rice. Gene. 1994; 141(2):215-20. DOI: 10.1016/0378-1119(94)90574-6. View

15.

Dennis E, Gerlach W, Walker J, Lavin M, Peacock W . Anaerobically regulated aldolase gene of maize. A chimaeric origin?. J Mol Biol. 1988; 202(4):759-67. DOI: 10.1016/0022-2836(88)90556-6. View

16.

Bialojan C, Takai A . Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics. Biochem J. 1988; 256(1):283-90. PMC: 1135400. DOI: 10.1042/bj2560283. View

17.

Kawamoto S, Hidaka H . 1-(5-Isoquinolinesulfonyl)-2-methylpiperazine (H-7) is a selective inhibitor of protein kinase C in rabbit platelets. Biochem Biophys Res Commun. 1984; 125(1):258-64. DOI: 10.1016/s0006-291x(84)80362-9. View