The Legionella Pneumophila Hel Locus Encodes Intracellularly Induced Homologs of Heavy-metal Ion Transporters of Alcaligenes Spp

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1996 May 1

PMID 8613357

Citations 10

Authors

M S McClain

M C Hurley

J K Brieland

N C Engleberg

Affiliations

Soon will be listed here.

Abstract

We continued characterization of the Legionella pneumophila hel locus. Mutagenesis and DNA sequencing identified three genes similar to the czc and cnr loci of Alcaligenes eutrophus and the ncc locus of Alcaligenes xylosoxidans. On the basis of their similarity to these loci, we designated the L. pneumophila genes helC, helB, and helA. Mutations in the hel genes led to reduced cytopathicity towards U937 cells, although the mutant strains did not appear defective in other assays of virulence. Transcription of the hel locus was induced by the intracellular environment but was not induced by any of a variety of in vitro stress conditions. The function of the hel gene products remains to be determined.

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