Oral Immunization with a Recombinant Cysteine-rich Section of the Entamoeba Histolytica Galactose-inhibitable Lectin Elicits an Intestinal Secretory Immunoglobulin A Response That Has in Vitro Adherence Inhibition Activity

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1996 Apr 1

PMID 8606122

Citations 10

Authors

D E Beving

C J Soong

J I Ravdin

Affiliations

Soon will be listed here.

Abstract

The LC3-encoded 52-kDa recombinant protein includes amino acids 758 to 1134 of the 170-kDa subunit of the galactose-inhibitable lectin. Oral immunization of BALB/c mice with the LC3-encoded protein and cholera holotoxin induced an intestinal secretory immunoglobulin A (IgA) response (P < 0.01 compared with the control). There was a negative correlation (P = 0.001) between intestinal anti-LC3 IgA and serum IgA and IgG antibody responses. Intestinal secretions from immunized mice completely inhibited the galactose-specific adherence of axenic trophozoites ot Chinese hamster ovary cells (P < 0.01).

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