Disease Resistance Conferred by Expression of a Gene Encoding H2O2-generating Glucose Oxidase in Transgenic Potato Plants

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1995 Sep 1

PMID 8589621

Citations 71

Authors

G Wu

B J Shortt

E B Lawrence

E B LEVINE

K. C. Fitzsimmons

D M Shah

Affiliations

Soon will be listed here.

Abstract

Plant defense responses to pathogen infection involve the production of active oxygen species, including hydrogen peroxide (H2O2). We obtained transgenic potato plants expressing a fungal gene encoding glucose oxidase, which generates H2O2 when glucose is oxidized. H2O2 levels were elevated in both leaf and tuber tissues of these plants. Transgenic potato tubers exhibited strong resistance to a bacterial soft rot disease caused by Erwinia carotovora subsp carotovora, and disease resistance was sustained under both aerobic and anaerobic conditions of bacterial infection. This resistance to soft rot was apparently mediated by elevated levels of H2O2, because the resistance could be counteracted by exogenously added H2O2-degrading catalase. The transgenic plants with increased levels of H2O2 also exhibited enhanced resistance to potato late blight caused by Phytophthora infestans. The development of lesions resulting from infection by P. infestans was significantly delayed in leaves of these plants. Thus, the expression of an active oxygen species-generating enzyme in transgenic plants represents a novel approach for engineering broad-spectrum disease resistance in plants.

Citing Articles

Proteomics fingerprinting reveals importance of iron and oxidative stress in - interactions.

Giroux L, Isayenka I, Lerat S, Beaudoin N, Beaulieu C Front Microbiol. 2024; 15:1466927.

PMID: 39417082 PMC: 11479980. DOI: 10.3389/fmicb.2024.1466927.

Enhanced activity of introduced in solarized soil and its implications on the integrated control of strawberry-black root rot.

Elshahawy I, Saied N, Abd-El-Kareem F, Abd-Elgawad M Heliyon. 2024; 10(17):e36795.

PMID: 39263098 PMC: 11387541. DOI: 10.1016/j.heliyon.2024.e36795.

CRISPR/Cas9-mediated knockout of Bsr-d1 enhances the blast resistance of rice in Northeast China.

Zhang Y, Lin X, Li L, Piao R, Wu S, Song A Plant Cell Rep. 2024; 43(4):100.

PMID: 38498220 DOI: 10.1007/s00299-024-03192-0.

Management of sunflower charcoal-rot and maize late-wilt diseases using the aqueous extract of vermicompost (vermitea) and environmental-safe biochar derivative (wood vinegar).

Darwesh O, Elshahawy I Sci Rep. 2023; 13(1):17387.

PMID: 37833470 PMC: 10575965. DOI: 10.1038/s41598-023-43974-2.

Identification of Alkaline Salt Tolerance Genes in L. by Transcriptome Analysis.

Xu Y, Tao S, Zhu Y, Zhang Q, Li P, Wang H Genes (Basel). 2022; 13(8).

PMID: 36011404 PMC: 9408751. DOI: 10.3390/genes13081493.

References

Apostol I, Heinstein P, Low P . Rapid Stimulation of an Oxidative Burst during Elicitation of Cultured Plant Cells : Role in Defense and Signal Transduction. Plant Physiol. 1989; 90(1):109-16. PMC: 1061684. DOI: 10.1104/pp.90.1.109. View

Degousee N, Triantaphylides C, Montillet J . Involvement of Oxidative Processes in the Signaling Mechanisms Leading to the Activation of Glyceollin Synthesis in Soybean (Glycine max). Plant Physiol. 1994; 104(3):945-952. PMC: 160692. DOI: 10.1104/pp.104.3.945. View

Okuda T, Matsuda Y, Yamanaka A, Sagisaka S . Abrupt increase in the level of hydrogen peroxide in leaves of winter wheat is caused by cold treatment. Plant Physiol. 1991; 97(3):1265-7. PMC: 1081153. DOI: 10.1104/pp.97.3.1265. View

Kriechbaum M, Heilmann H, Wientjes F, Hahn M, Jany K, GASSEN H . Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3. FEBS Lett. 1989; 255(1):63-6. DOI: 10.1016/0014-5793(89)81061-0. View

Sanger M, Daubert S, Goodman R . Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter. Plant Mol Biol. 1990; 14(3):433-43. DOI: 10.1007/BF00028779. View

Vianello A, Macri F . Generation of superoxide anion and hydrogen peroxide at the surface of plant cells. J Bioenerg Biomembr. 1991; 23(3):409-23. DOI: 10.1007/BF00771012. View

Bradley D, Kjellbom P, Lamb C . Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response. Cell. 1992; 70(1):21-30. DOI: 10.1016/0092-8674(92)90530-p. View

Klessig D, Malamy J . The salicylic acid signal in plants. Plant Mol Biol. 1994; 26(5):1439-58. DOI: 10.1007/BF00016484. View

Keen N . The molecular biology of disease resistance. Plant Mol Biol. 1992; 19(1):109-22. DOI: 10.1007/BF00015609. View

10.

Heineke D, Sonnewald U, Bussis D, Gunter G, Leidreiter K, Wilke I . Apoplastic expression of yeast-derived invertase in potato : effects on photosynthesis, leaf solute composition, water relations, and tuber composition. Plant Physiol. 1992; 100(1):301-8. PMC: 1075552. DOI: 10.1104/pp.100.1.301. View

11.

Richins R, Scholthof H, Shepherd R . Sequence of figwort mosaic virus DNA (caulimovirus group). Nucleic Acids Res. 1987; 15(20):8451-66. PMC: 306370. DOI: 10.1093/nar/15.20.8451. View

12.

Chen Z, Silva H, Klessig D . Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. Science. 1993; 262(5141):1883-6. DOI: 10.1126/science.8266079. View

13.

Dixon R, Harrison M . Activation, structure, and organization of genes involved in microbial defense in plants. Adv Genet. 1990; 28:165-234. DOI: 10.1016/s0065-2660(08)60527-1. View

14.

Gallo L . Sterol ester hydrolase from rat pancreas. Methods Enzymol. 1981; 71 Pt C:664-74. DOI: 10.1016/0076-6879(81)71079-6. View

15.

Mehdy M . Active Oxygen Species in Plant Defense against Pathogens. Plant Physiol. 1994; 105(2):467-472. PMC: 159383. DOI: 10.1104/pp.105.2.467. View

16.

Levine A, Tenhaken R, Dixon R, Lamb C . H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell. 1994; 79(4):583-93. DOI: 10.1016/0092-8674(94)90544-4. View

17.

Schreck R, Rieber P, Baeuerle P . Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1. EMBO J. 1991; 10(8):2247-58. PMC: 452914. DOI: 10.1002/j.1460-2075.1991.tb07761.x. View

18.

Brennan T, Frenkel C . Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiol. 1977; 59(3):411-6. PMC: 542414. DOI: 10.1104/pp.59.3.411. View

19.

Bowles D . Defense-related proteins in higher plants. Annu Rev Biochem. 1990; 59:873-907. DOI: 10.1146/annurev.bi.59.070190.004301. View

20.

Lamb C, Lawton M, Dron M, Dixon R . Signals and transduction mechanisms for activation of plant defenses against microbial attack. Cell. 1989; 56(2):215-24. DOI: 10.1016/0092-8674(89)90894-5. View