Evaluation of E-Test for Determination of Antimicrobial MICs for Pseudomonas Aeruginosa Isolates from Cystic Fibrosis Patients

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1995 Dec 1

PMID 8586700

Citations 9

Authors

E F Marley

C Mohla

J M Campos

Affiliations

Soon will be listed here.

Abstract

We determined the E-Test and National Committee for Clinical Laboratory Standards standardized agar dilution MICs of ceftazidime, ciprofloxacin, piperacillin, and tobramycin for Pseudomonas aeruginosa during tests of 100 rough and mucoid P. aeruginosa isolates from cystic fibrosis patients. The levels of agreement (+/- 1 log2 dilution) between quantitative E-Test and agar dilution MIC results were 80, 97, 73, and 89% for ceftazidime, ciprofloxacin, piperacillin, and tobramycin, respectively. Comparison of the results after converting the MIC data to qualitative categories (susceptible, intermediate, and resistant) yielded levels of agreement of 84, 96, 88, and 93% for the same agents, respectively. Of the 39 qualitative discrepancies, 36 were minor and 3 were very major. We conclude that use of the E-Test is easier and more practical than use of the agar dilution method for most laboratories and that the E-Test furnishes results which are at least as accurate as those obtained by the agar dilution method. However, the higher cost of the E-Test method would likely discourage most laboratories from selecting it over disk diffusion for routine antimicrobial susceptibility testing of P. aeruginosa isolates from cystic fibrosis patients.

Citing Articles

Long-term evolution of antibiotic tolerance in lung infections.

Ghoul M, Andersen S, Marvig R, Johansen H, Jelsbak L, Molin S Evol Lett. 2023; 7(6):389-400.

PMID: 38045720 PMC: 10693005. DOI: 10.1093/evlett/qrad034.

Changes in toxin production of environmental Pseudomonas aeruginosa isolates exposed to sub-inhibitory concentrations of three common antibiotics.

Mojsoska B, Ghoul M, Perron G, Jenssen H, Alatraktchi F PLoS One. 2021; 16(3):e0248014.

PMID: 33662048 PMC: 7932067. DOI: 10.1371/journal.pone.0248014.

Development, optimization, standardization, and validation of a simple in-house agar gradient method to determine minimum inhibitory concentration of vancomycin for .

Rai S, Tandon M, Singh N, Manchanda V, Kaur I J Lab Physicians. 2019; 11(3):220-228.

PMID: 31579244 PMC: 6771310. DOI: 10.4103/JLP.JLP_11_19.

Tobramycin and Amikacin Delay Adhesion and Microcolony Formation in Cystic Fibrosis Isolates.

Olivares E, Badel-Berchoux S, Provot C, Jaulhac B, Prevost G, Bernardi T Front Microbiol. 2017; 8:1289.

PMID: 28744274 PMC: 5504095. DOI: 10.3389/fmicb.2017.01289.

Porin involvement in cephalosporin and carbapenem resistance of Burkholderia pseudomallei.

Aunkham A, Schulte A, Winterhalter M, Suginta W PLoS One. 2014; 9(5):e95918.

PMID: 24788109 PMC: 4006797. DOI: 10.1371/journal.pone.0095918.

References

GEORGE R . Pseudomonas infections in cystic fibrosis. Arch Dis Child. 1987; 62(5):438-9. PMC: 1778407. DOI: 10.1136/adc.62.5.438. View

Colombo A, Barchiesi F, McGough D, Rinaldi M . Comparison of Etest and National Committee for Clinical Laboratory Standards broth macrodilution method for azole antifungal susceptibility testing. J Clin Microbiol. 1995; 33(3):535-40. PMC: 227986. DOI: 10.1128/jcm.33.3.535-540.1995. View

Levy J . Antibiotic therapy in cystic fibrosis. Evaluation of efficacy. Chest. 1988; 94(2 Suppl):150S-156S. View

ODonnell C, Bader M, Zibrak J, Jensen W, Rose R . Abnormal airway function in individuals with the acquired immunodeficiency syndrome. Chest. 1988; 94(5):945-8. DOI: 10.1378/chest.94.5.945. View

Steinkamp G, Tummler B, Malottke R, von der Hardt H . Treatment of pseudomonas aeruginosa colonisation in cystic fibrosis. Arch Dis Child. 1989; 64(7):1022-8. PMC: 1792706. DOI: 10.1136/adc.64.7.1022. View

Baker C, Stocker S, Culver D, Thornsberry C . Comparison of the E Test to agar dilution, broth microdilution, and agar diffusion susceptibility testing techniques by using a special challenge set of bacteria. J Clin Microbiol. 1991; 29(3):533-8. PMC: 269813. DOI: 10.1128/jcm.29.3.533-538.1991. View

Korvick J, Yu V . Antimicrobial agent therapy for Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1991; 35(11):2167-72. PMC: 245354. DOI: 10.1128/AAC.35.11.2167. View

Rautelin H, Renkonen I, RENKONEN O . Evaluation of the E test in testing susceptibility of Pseudomonas aeruginosa to tobramycin. Eur J Clin Microbiol Infect Dis. 1992; 11(2):177-80. DOI: 10.1007/BF01967074. View

Joyce L, DOWNES J, Stockman K, Andrew J . Comparison of five methods, including the PDM Epsilometer test (E test), for antimicrobial susceptibility testing of Pseudomonas aeruginosa. J Clin Microbiol. 1992; 30(10):2709-13. PMC: 270503. DOI: 10.1128/jcm.30.10.2709-2713.1992. View

10.

Wust J, Hardegger U . Comparison of the E test and a reference agar dilution method for susceptibility testing of anaerobic bacteria. Eur J Clin Microbiol Infect Dis. 1992; 11(12):1169-73. DOI: 10.1007/BF01961139. View

11.

Couroux P, Massey V, Schieven B, Lannigan R, Hussain Z . Comparison of the E-test and reference agar dilution method for susceptibility of gram-negative anaerobic organisms. Am J Clin Pathol. 1993; 100(3):301-3. DOI: 10.1093/ajcp/100.3.301. View

12.

Schulz J, Sahm D . Reliability of the E test for detection of ampicillin, vancomycin, and high-level aminoglycoside resistance in Enterococcus spp. J Clin Microbiol. 1993; 31(12):3336-9. PMC: 266427. DOI: 10.1128/jcm.31.12.3336-3339.1993. View

13.

Jorgensen J, Ferraro M, McElmeel M, Spargo J, Swenson J, Tenover F . Detection of penicillin and extended-spectrum cephalosporin resistance among Streptococcus pneumoniae clinical isolates by use of the E test. J Clin Microbiol. 1994; 32(1):159-63. PMC: 262988. DOI: 10.1128/jcm.32.1.159-163.1994. View

14.

Macias E, Mason Jr E, Ocera H, Larocco M . Comparison of E test with standard broth microdilution for determining antibiotic susceptibilities of penicillin-resistant strains of Streptococcus pneumoniae. J Clin Microbiol. 1994; 32(2):430-2. PMC: 263048. DOI: 10.1128/jcm.32.2.430-432.1994. View

15.

Van Dyck E, Smet H, Piot P . Comparison of E test with agar dilution for antimicrobial susceptibility testing of Neisseria gonorrhoeae. J Clin Microbiol. 1994; 32(6):1586-8. PMC: 264044. DOI: 10.1128/jcm.32.6.1586-1588.1994. View

16.

Hoffner S, Klintz L, Olsson-Liljequist B, Bolmstrom A . Evaluation of Etest for rapid susceptibility testing of Mycobacterium chelonae and M. fortuitum. J Clin Microbiol. 1994; 32(8):1846-9. PMC: 263889. DOI: 10.1128/jcm.32.8.1846-1849.1994. View

17.

DOUGLAS C, Siarakas S, Gottlieb T . Evaluation of E test as a rapid method for determining MICs for nutritionally variant streptococci. J Clin Microbiol. 1994; 32(9):2318-20. PMC: 263995. DOI: 10.1128/jcm.32.9.2318-2320.1994. View

18.

Michel B . Antibacterial therapy in cystic fibrosis. A review of the literature published between 1980 and February 1987. Chest. 1988; 94(2 Suppl):129S-140S. DOI: 10.1378/chest.94.2.129s. View