Propagation of Infectious Salmon Anaemia (ISA) Virus in Cell Culture

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 1995 Jan 1

PMID 8581019

Citations 15

Authors

B H Dannevig

K Falk

C M Press

Affiliations

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Abstract

A long-term cell line supporting growth of the infectious salmon anaemia (ISA) virus has been established. The cell line (SHK-1) was developed from a culture of head kidney leucocytes from Atlantic salmon, and exhibited macrophage-like enzyme reactivities. By means of transmission experiments, ISA infectivity of cell culture medium could be demonstrated from day 5 after infection of SHK-1 cells with ISA-infective tissue homogenate. ISA infectivity of cell culture medium increased following repeated passages of virus. ISA-infected cell cultures develop cytopathic effects (CPE), making quantitation of virus possible. The development of CPE in ISA virus infected cells was inhibited by ammonium chloride, chloroquine and bafilomycin A, suggesting that infection of SHK-1 cells with ISA virus requires a low-pH step.

Citing Articles

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Selective Stimulation of Duplicated Atlantic Salmon MHC Pathway Genes by Interferon-Gamma.

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Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology.

Hoare R, K D Thompson , Herath T, Collet B, Bron J, Adams A PLoS One. 2016; 11(7):e0159155.

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