Detection and Genotyping of Herpes Simplex Virus Types 1 and 2 by Polymerase Chain Reaction

Overview

Journal Mol Cell Probes

Specialties Biotechnology
Cell Biology
Molecular Biology

Date 1995 Oct 1

PMID 8569766

Citations 7

Authors

G Lucotte

C Bathelier

V Lespiaux

C Bali

T Champenois

Affiliations

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Abstract

A simple and rapid polymerase chain reaction (PCR) procedure was developed for simultaneous detection and typing of herpes simplex virus (HSV) types 1 and 2. It was possible to detect and type HSV using two primers pairs in a simultaneous double PCR reaction, where the type of HSV present was determined on the basis of an ethidium-bromide-stained band after agarose gel electrophoresis. This PCR assay was tested on about 500 clinical specimens.

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