Improved Strategy for Mutation Detection--a Modification to the Enzyme Mismatch Cleavage Method

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1995 Dec 25

PMID 8559669

Citations 4

Authors

J J Babon

R Youil

R G Cotton

Affiliations

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Singh K, Evens H, Nair N, Rincon M, Sarcar S, Samara-Kuko E Mol Ther. 2018; 26(5):1241-1254.

PMID: 29599079 PMC: 5993986. DOI: 10.1016/j.ymthe.2018.02.023.

Comparison of T7E1 and surveyor mismatch cleavage assays to detect mutations triggered by engineered nucleases.

Vouillot L, Thelie A, Pollet N G3 (Bethesda). 2015; 5(3):407-15.

PMID: 25566793 PMC: 4349094. DOI: 10.1534/g3.114.015834.

The use of resolvases T4 endonuclease VII and T7 endonuclease I in mutation detection.

Babon J, McKenzie M, Cotton R Mol Biotechnol. 2003; 23(1):73-81.

PMID: 12611271 DOI: 10.1385/MB:23:1:73.

Detection of all single-base mismatches in solution by chemiluminescence.

Nelson N, Hammond P, Matsuda E, Goud A, Becker M Nucleic Acids Res. 1996; 24(24):4998-5003.

PMID: 9016672 PMC: 146343. DOI: 10.1093/nar/24.24.4998.

References

Youil R, Kemper B, Cotton R . Screening for mutations by enzyme mismatch cleavage with T4 endonuclease VII. Proc Natl Acad Sci U S A. 1995; 92(1):87-91. PMC: 42822. DOI: 10.1073/pnas.92.1.87. View

Pottmeyer S, Kemper B . T4 endonuclease VII resolves cruciform DNA with nick and counter-nick and its activity is directed by local nucleotide sequence. J Mol Biol. 1992; 223(3):607-15. DOI: 10.1016/0022-2836(92)90977-r. View