D-histidine Utilization in Salmonella Typhimurium is Controlled by the Leucine-responsive Regulatory Protein (Lrp)

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1996 Jan 1

PMID 8550449

Citations 9

Authors

K Hecht

S Zhang

T Klopotowski

G F AMES

Affiliations

Soon will be listed here.

Abstract

A new class of D-histidine-utilizing mutants which carry mutations in the gene encoding the leucine-responsive regulatory protein (Lrp) has been identified in Salmonella typhimurium. The lrp mutations arise as suppressors of mutations in the genes encoding the histidine permease which drastically decrease the level of histidine transport activity. However, the suppressor effect is not exerted by elevating the level of the permease. Rather, the properties of the suppressor mutants are consistent with the notion that the parent permease mutants transport D-histidine at a low level and that in the suppressor mutants D-histidine is utilized effectively through elevated levels of racemization. The enzymatic activity of D-alanine dehydrogenase (Dad) is shown to be elevated in the suppressor mutants and is a possible pathway of D-histidine utilization. The suppressor mutations are located in the helix-turn-helix region of Lrp.

Citing Articles

Evidence from Mutational Analysis for a Single Transmembrane Substrate Binding Site in the Histidine ATP-Binding Cassette Transporter of Salmonella enterica Serovar Typhimurium.

Heuveling J, Landmesser H, Schneider E J Bacteriol. 2018; 201(2).

PMID: 30348830 PMC: 6304659. DOI: 10.1128/JB.00521-18.

The Bacterial Two-Hybrid System Uncovers the Involvement of Acetylation in Regulating of Lrp Activity in Typhimurium.

Qin R, Sang Y, Ren J, Zhang Q, Li S, Cui Z Front Microbiol. 2016; 7:1864.

PMID: 27909434 PMC: 5112231. DOI: 10.3389/fmicb.2016.01864.

Unexpected coregulator range for the global regulator Lrp of Escherichia coli and Proteus mirabilis.

Hart B, Blumenthal R J Bacteriol. 2010; 193(5):1054-64.

PMID: 21169483 PMC: 3067584. DOI: 10.1128/JB.01183-10.

Leucine-responsive regulatory protein (Lrp) acts as a virulence repressor in Salmonella enterica serovar Typhimurium.

Baek C, Wang S, Roland K, Curtiss 3rd R J Bacteriol. 2008; 191(4):1278-92.

PMID: 19074398 PMC: 2631999. DOI: 10.1128/JB.01142-08.

Comparison of DeltarelA strains of Escherichia coli and Salmonella enterica serovar Typhimurium suggests a role for ppGpp in attenuation regulation of branched-chain amino acid biosynthesis.

Tedin K, Norel F J Bacteriol. 2001; 183(21):6184-96.

PMID: 11591661 PMC: 100096. DOI: 10.1128/JB.183.21.6184-6196.2001.

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