Carbonic Anhydrase is Present in Human Oesophageal Epithelium and Submucosal Glands

Overview

Journal Histochem J

Specialty Biochemistry

Date 1995 Aug 1

PMID 8550377

Citations 3

Authors

K N Christie

C Thomson

S Morley

J Anderson

D Hopwood

Affiliations

Soon will be listed here.

Abstract

Carbonic anhydrase (EC 4.2.1.1) activity was investigated in normal human oesophageal mucosa using the Hansson and Ridderstråle catalytic cobalt methods. The enzyme was detected in the cell membranes and nuclei and, to a lesser extent, in the cytoplasm of the epithelial cells of the mucosa giving a 'chicken wire' appearance. Activity decreased towards the lumen. Other stratified squamous epithelia--buccal mucosa, ectocervix and skin--gave a similar pattern. Acinar cells of oesophageal submucosal glands also exhibited activity for the enzyme, but the ducts did not. The formation of reaction product was prevented by acetazolamide and ethoxzolamide and by the omission of bicarbonate frm the substrate medium. Carbonic anhydrase in oesophageal squamous epithelium may be involved in the control of intra- and extracellular pH, while that in the glands is more likely to be concerned with bicarbonate secretion.

Citing Articles

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Durkes A, Sivasankar M Ann Otol Rhinol Laryngol. 2014; 123(1):71-6.

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The distribution of carbonic anhydrase II in human, pig and rat oesophageal epithelium.

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Human oesophageal bicarbonate secretion: a phenomenon waiting for a role.

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