A COS-cell-based System for Rapid Production and Quantification of ScFv::IgC Kappa Antibody Fragments

Overview

Journal Gene

Specialty Molecular Biology

Date 1995 Dec 12

PMID 8543174

Citations 6

Authors

R Ridder

S Geisse

B Kleuser

P Kawalleck

H Gram

Affiliations

Soon will be listed here.

Abstract

Single-chain Fv (scFv) antibody (Ab) fragments were transiently produced in COS-1 cells utilizing a mammalian expression vector featuring a murine immunoglobulin (Ig) light-chain leader sequence for efficient secretion and a murine Ig kappa constant domain (IgC kappa) for detection. Several hundred milliliters of supernatants from large-scale COS cell transfections were sufficient to purify the scFv::IgC kappa fusion proteins by one-step affinity chromatography utilizing an immobilized rat anti-mouse IgC kappa monoclonal Ab. Furthermore, the murine IgC kappa domain allowed for accurate quantification of the scFv::IgC kappa fusion protein secreted into the COS cell supernatant by a sandwich enzyme-linked immunosorbent assay (S-ELISA).

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