Enzyme Immunoassay for Detection of Antibodies Against Isolated Flagella of Legionella Pneumophila

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 1995 Sep 1

PMID 8536723

Citations 2

Authors

B Neumeister

M Susa

B Nowak

E Straube

G Ruckdeschel

J Hacker

R Marre

Affiliations

Soon will be listed here.

Abstract

Flagella of Legionella pneumophila serogroup 1, strain Philadelphia (ATCC 33152), were isolated and used as antigen to detect antibodies by enzyme immunoassay (EIA) in sera of patients with significant titers against Legionella as determined by the indirect immunofluorescence assay (IFA). Healthy blood donors served as a control group. Whereas the sera of blood donors were negative in both assays, sera of patients showed two patterns of reactivity. Sera with elevated titers against Legionella pneumophila serogroup 1 or Legionella gormanii in IFA usually demonstrated high titers in EIA. However, high IFA titers against Legionella bozemanii were associated with low titers in EIA. The data show that flagella protein of Legionella pneumophila serogroup 1 strain Philadelphia is of limited value for the detection of antibodies against Legionella other than Legionella pneumophila serogroup 1, although in vitro studies revealed genus-specific epitopes. This might be due to unpredictable variabilities in the expression of flagella in vivo.

Citing Articles

Legionella and Legionnaires' disease: 25 years of investigation.

Fields B, Benson R, Besser R Clin Microbiol Rev. 2002; 15(3):506-26.

PMID: 12097254 PMC: 118082. DOI: 10.1128/CMR.15.3.506-526.2002.

Characterization of Bartonella clarridgeiae flagellin (FlaA) and detection of antiflagellin antibodies in patients with lymphadenopathy.

Sander A, Zagrosek A, BREDT W, Schiltz E, Piemont Y, Lanz C J Clin Microbiol. 2000; 38(8):2943-8.

PMID: 10921956 PMC: 87154. DOI: 10.1128/JCM.38.8.2943-2948.2000.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Harrison T, Dournon E, Taylor A . Evaluation of sensitivity of two serological tests for diagnosing pneumonia caused by Legionella pneumophila serogroup 1. J Clin Pathol. 1987; 40(1):77-82. PMC: 1140833. DOI: 10.1136/jcp.40.1.77. View

Rello J, Quintana E, Ausina V, Net A, Prats G . A three-year study of severe community-acquired pneumonia with emphasis on outcome. Chest. 1993; 103(1):232-5. DOI: 10.1378/chest.103.1.232. View

CHANDLER F, Roth I, CALLAWAY C, Bump J, Thomason B, Weaver R . Flagella on Legionnaires' disease bacteria: ultastructural observations. Ann Intern Med. 1980; 93(5):711-4. DOI: 10.7326/0003-4819-93-5-711. View

WILKINSON H, Fikes B, Cruce D . Indirect immunofluorescence test for serodiagnosis of Legionnaires disease: evidence for serogroup diversity of Legionnaires disease bacterial antigens and for multiple specificity of human antibodies. J Clin Microbiol. 1979; 9(3):379-83. PMC: 273034. DOI: 10.1128/jcm.9.3.379-383.1979. View

Rodgers F, Greaves P, MACRAE A, Lewis M . Electron microscopic evidence of flagella and pili on Legionella pneumophila. J Clin Pathol. 1980; 33(12):1184-8. PMC: 1146372. DOI: 10.1136/jcp.33.12.1184. View

Heuner K, Brand B, Luck P, Mann K, Marre R, Ott M . Cloning and genetic characterization of the flagellum subunit gene (flaA) of Legionella pneumophila serogroup 1. Infect Immun. 1995; 63(7):2499-507. PMC: 173334. DOI: 10.1128/iai.63.7.2499-2507.1995. View

Rodgers F, Greaves P, MACRAE A . Flagella and fimbriae on Legionella organisms. Lancet. 1979; 2(8145):753-4. DOI: 10.1016/s0140-6736(79)90691-3. View

Bornstein N, Marmet D, Dumaine M, Surgot M, Fleurette J . Detection of flagella in 278 Legionella strains by latex reagent sensitized with antiflagellum immunoglobulins. J Clin Microbiol. 1991; 29(5):953-6. PMC: 269914. DOI: 10.1128/jcm.29.5.953-956.1991. View

10.

Hoge C, Breiman R . Advances in the epidemiology and control of Legionella infections. Epidemiol Rev. 1991; 13:329-40. DOI: 10.1093/oxfordjournals.epirev.a036076. View

11.

CHANDLER F, Thomason B, HEBERT G . Flagella on Legionnaires' disease bacteria in the human lung. Ann Intern Med. 1980; 93(5):715-6. DOI: 10.7326/0003-4819-93-5-715. View

12.

Lowry P, Tompkins L . Nosocomial legionellosis: a review of pulmonary and extrapulmonary syndromes. Am J Infect Control. 1993; 21(1):21-7. DOI: 10.1016/0196-6553(93)90203-g. View

13.

Thomason B, CHANDLER F, Hollis D . Flagella on Legionnaires' disease bacteria: an interim report. Ann Intern Med. 1979; 91(2):224-6. DOI: 10.7326/0003-4819-91-2-224. View

14.

Elliott J, Johnson W . Immunological and biochemical relationships among flagella isolated from Legionella pneumophila serogroups 1, 2, and 3. Infect Immun. 1981; 33(2):602-10. PMC: 350741. DOI: 10.1128/iai.33.2.602-610.1981. View

15.

Ott M, Messner P, Heesemann J, Marre R, Hacker J . Temperature-dependent expression of flagella in Legionella. J Gen Microbiol. 1991; 137(8):1955-61. DOI: 10.1099/00221287-137-8-1955. View

16.

Craft J, Fischer D, Shimamoto G, Steere A . Antigens of Borrelia burgdorferi recognized during Lyme disease. Appearance of a new immunoglobulin M response and expansion of the immunoglobulin G response late in the illness. J Clin Invest. 1986; 78(4):934-9. PMC: 423723. DOI: 10.1172/JCI112683. View