Molecular Cloning, Characterization and Expression of an Elongation Factor 1 Alpha Gene in Maize

Overview

Journal Plant Mol Biol

Date 1995 Nov 1

PMID 8534856

Citations 26

Authors

T Berberich

K Sugawara

M Harada

T Kusano

Affiliations

Soon will be listed here.

Abstract

A cDNA (zmEF1A) and the corresponding genomic clone (zmgEF1A) of a member of the gene family encoding the alpha subunit of translation elongation factor 1 (EF-1 alpha) have been isolated from maize. The deduced amino acid sequence is 447 residues long interrupted by one intron. Southern blot analysis reveals that the cloned EF-1 alpha gene is one member out of a family consisting of at least six genes. As shown by northern hybridizations in leaves the mRNA level increases at low temperature whereas time-course experiments over 24 h at 5 degrees C show that in roots the overall mRNA level of EF-1 alpha is transiently decreased. These results indicate that the expression of EF-1 alpha is differently regulated in leaves and roots under cold stress.

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