Antisense Oligodeoxynucleotide Inhibition of Delta Protein Kinase C Expression Accelerates Induced Differentiation of Murine Erythroleukaemia Cells

Overview

Journal Biochem J

Specialty Biochemistry

Date 1995 Dec 1

PMID 8526869

Citations 6

Authors

A Pessino

M Passalacqua

B Sparatore

M Patrone

E Melloni

S Pontremoli

Affiliations

Soon will be listed here.

Abstract

The potential regulatory role of delta protein kinase C (delta PKC) in murine erythroleukaemia cell differentiation was studied by using antisense oligodeoxynucleotides targeting the translation initiation region of mouse delta PKC mRNA. Cell treatment with antisense oligonucleotides, at a concentration of 20 microM, followed by hexamethylenebisacetamide induction, produced a specific 2-fold increase in the differentiation rate of both slowly and rapidly differentiating murine erythroleukaemia cell clones. Cell permeabilization by a cationic lipid resulted in a decrease of one order of magnitude in the amounts of antisense oligonucleotides necessary to elicit the maximal response, and accelerated the kinetics of the stimulatory effect. These changes in murine erythroleukaemia cell differentiation rates, observed in both cell clones, were associated with 60% and 50% decreases, respectively, in delta PKC immunoreactive protein in slowly and rapidly differentiating cells. The present results indicate strongly that basal levels of delta PKC in murine erythroleukaemia cells are essential in regulating the initial differentiation rate of these cells in response to chemical induction, and provide further evidence that this PKC isoform plays a fundamental role in maintaining the undifferentiated phenotype of murine erythroleukaemia cells.

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References

Friend C, Scher W, Holland J, Sato T . Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971; 68(2):378-82. PMC: 388942. DOI: 10.1073/pnas.68.2.378. View

Pessino A, Sparatore B, Patrone M, Passalacqua M, Melloni E, Pontremoli S . Differential expression of protein kinase C isoform genes in three murine erythroleukemia cell variants: implication for chemical induced differentiation. Biochem Biophys Res Commun. 1994; 204(2):461-7. DOI: 10.1006/bbrc.1994.2482. View

Reuben R, Wife R, Breslow R, Rifkind R, Marks P . A new group of potent inducers of differentiation in murine erythroleukemia cells. Proc Natl Acad Sci U S A. 1976; 73(3):862-6. PMC: 336019. DOI: 10.1073/pnas.73.3.862. View

Melloni E, Pontremoli S, Michetti M, Sacco O, Cakiroglu A, Jackson J . Protein kinase C activity and hexamethylenebisacetamide-induced erythroleukemia cell differentiation. Proc Natl Acad Sci U S A. 1987; 84(15):5282-6. PMC: 298839. DOI: 10.1073/pnas.84.15.5282. View

Melloni E, Pontremoli S, Viotti P, Patrone M, Marks P, Rifkind R . Differential expression of protein kinase C isozymes and erythroleukemia cell differentiation. J Biol Chem. 1989; 264(31):18414-8. View

Hashimoto K, Kishimoto A, Aihara H, Yasuda I, Mikawa K, NISHIZUKA Y . Protein kinase C during differentiation of human promyelocytic leukemia cell line, HL-60. FEBS Lett. 1990; 263(1):31-4. DOI: 10.1016/0014-5793(90)80698-i. View

Melloni E, Pontremoli S, Sparatore B, Patrone M, Grossi F, Marks P . Introduction of the beta isozyme of protein kinase C accelerates induced differentiation of murine erythroleukemia cells. Proc Natl Acad Sci U S A. 1990; 87(12):4417-20. PMC: 54125. DOI: 10.1073/pnas.87.12.4417. View

Mischak H, Bodenteich A, Kolch W, Goodnight J, Hofer F, MUSHINSKI J . Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein. Biochemistry. 1991; 30(32):7925-31. DOI: 10.1021/bi00246a008. View

Chiang M, Chan H, Zounes M, Freier S, Lima W, Bennett C . Antisense oligonucleotides inhibit intercellular adhesion molecule 1 expression by two distinct mechanisms. J Biol Chem. 1991; 266(27):18162-71. View

10.

Bock L, Griffin L, Latham J, Vermaas E, Toole J . Selection of single-stranded DNA molecules that bind and inhibit human thrombin. Nature. 1992; 355(6360):564-6. DOI: 10.1038/355564a0. View

11.

Ogita K, Miyamoto S, Yamaguchi K, Koide H, Fujisawa N, Kikkawa U . Isolation and characterization of delta-subspecies of protein kinase C from rat brain. Proc Natl Acad Sci U S A. 1992; 89(5):1592-6. PMC: 48498. DOI: 10.1073/pnas.89.5.1592. View

12.

Borner C, Guadagno S, Hsiao W, Fabbro D, Barr M, Weinstein I . Expression of four protein kinase C isoforms in rat fibroblasts. Differential alterations in ras-, src-, and fos-transformed cells. J Biol Chem. 1992; 267(18):12900-10. View

13.

Watanabe T, Ono Y, Taniyama Y, Hazama K, Igarashi K, Ogita K . Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies. Proc Natl Acad Sci U S A. 1992; 89(21):10159-63. PMC: 50297. DOI: 10.1073/pnas.89.21.10159. View

14.

Crooke S . Therapeutic applications of oligonucleotides. Biotechnology (N Y). 1992; 10(8):882-6. DOI: 10.1038/nbt0892-882. View

15.

Colige A, Sokolov B, Nugent P, Baserga R, Prockop D . Use of an antisense oligonucleotide to inhibit expression of a mutated human procollagen gene (COL1A1) in transfected mouse 3T3 cells. Biochemistry. 1993; 32(1):7-11. DOI: 10.1021/bi00052a002. View

16.

Stein C, Tonkinson J, Zhang L, Yakubov L, Gervasoni J, TAUB R . Dynamics of the internalization of phosphodiester oligodeoxynucleotides in HL60 cells. Biochemistry. 1993; 32(18):4855-61. DOI: 10.1021/bi00069a022. View

17.

Sparatore B, Pessino A, Patrone M, Passalacqua M, Melloni E, Pontremoli S . Role of delta-PKC on the differentiation process of murine erythroleukemia cells. Biochem Biophys Res Commun. 1993; 193(1):220-7. DOI: 10.1006/bbrc.1993.1612. View

18.

Stein C, Cheng Y . Antisense oligonucleotides as therapeutic agents--is the bullet really magical?. Science. 1993; 261(5124):1004-12. DOI: 10.1126/science.8351515. View

19.

Mischak H, PIERCE J, Goodnight J, Kazanietz M, Blumberg P, MUSHINSKI J . Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta. J Biol Chem. 1993; 268(27):20110-5. View

20.

Denning M, Dlugosz A, Howett M, Yuspa S . Expression of an oncogenic rasHa gene in murine keratinocytes induces tyrosine phosphorylation and reduced activity of protein kinase C delta. J Biol Chem. 1993; 268(35):26079-81. View