Gamma Delta Lymphocytes in Endocrine Autoimmunity: Evidence of Expansion in Graves' Disease but Not in Type 1 Diabetes

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1993 May 1

PMID 8485915

Citations 9

Authors

L Alcalde

F Vargas

E Tolosa

G Obiols

M FOZ

D Jaraquemada

R Pujol-Borrell

Affiliations

Soon will be listed here.

Abstract

Endocrine autoimmune disorders are mediated by T cell-dependent responses to organ-specific antigens, but the mechanisms initiating the process remain unknown. Lymphocytes which use the gamma delta heterodimer as T cell receptor (TCR) for antigen constitute a distinct subset of T cells whose function remains elusive. In order to investigate their possible involvement in endocrine autoimmunity we have determined the proportion of gamma delta T cells in the peripheral blood of 23 patients with type 1 (insulin-dependent) diabetes mellitus (type-1 DM) and 30 patients with autoimmune thyrotoxicosis (Graves' disease). T lymphocyte TCR expression was assessed by fluorescence-activated flow cytometry on peripheral blood mononuclear cells using MoAbs UCHT1 (CD3), TCR delta 1 (gamma delta TCR), WT31 and beta F1 (alpha beta TCR) and both the percentage of T cells expressing gamma delta and the ratio gamma delta/alpha beta were calculated. In the diabetic patients gamma delta cells were not significantly different from the control group (7.7 +/- 54% versus 8.0 +/- 5.5% of T cells, P NS). There was no relation between the proportion of gamma delta lymphocytes and the presence of islet cell antibodies (ICA) in the sera. The Graves' patients showed a tendency towards a higher proportion of gamma delta T lymphocytes than the controls (gamma delta/alpha beta ratios: 0.095 +/- 0.047 versus 0.063 +/- 0.022, P = 0.03). In 14 Graves' patients the number of gamma delta were measured in paired samples of peripheral and intrathyroidal lymphocytes, demonstrating an expansion of gamma delta within the thyroid glands (0.21 +/- 0.3 versus 0.095 +/- 0.047, P = 0.032). Immunohistochemical studies showed that gamma delta cells were scattered among the predominant alpha beta lymphocytes infiltrating the thyroid gland and that they account for 10% of intraepithelial lymphocytes. No relation was found between the increase of gamma delta lymphocytes and any clinical features.

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References

Bottazzo G, Florin-Christensen A, Doniach D . Islet-cell antibodies in diabetes mellitus with autoimmune polyendocrine deficiencies. Lancet. 1974; 2(7892):1279-83. DOI: 10.1016/s0140-6736(74)90140-8. View

Tolosa E, Roura C, Catalfamo M, Marti M, Lucas-Martin A, Sanmarti A . Expression of intercellular adhesion molecule-1 in thyroid follicular cells in autoimmune, non-autoimmune and neoplastic diseases of the thyroid gland: discordance with HLA. J Autoimmun. 1992; 5(1):107-18. DOI: 10.1016/s0896-8411(05)80055-1. View

Todd I, Pujol-Borrell R, Hammond L, Bottazzo G, Feldmann M . Interferon-gamma induces HLA-DR expression by thyroid epithelium. Clin Exp Immunol. 1985; 61(2):265-73. PMC: 1577305. View

Band H, Hochstenbach F, McLean J, Hata S, Krangel M, Brenner M . Immunochemical proof that a novel rearranging gene encodes the T cell receptor delta subunit. Science. 1987; 238(4827):682-4. DOI: 10.1126/science.3672118. View

Lucas-Martin A, Todd I, Bottazzo G, Pujol-Borrell R . Occurrence of thyrocyte HLA class II expression in a wide variety of thyroid diseases: relationship with lymphocytic infiltration and thyroid autoantibodies. J Clin Endocrinol Metab. 1988; 66(2):367-75. DOI: 10.1210/jcem-66-2-367. View

Havran W, Allison J . Developmentally ordered appearance of thymocytes expressing different T-cell antigen receptors. Nature. 1988; 335(6189):443-5. DOI: 10.1038/335443a0. View

Bonneville M, Janeway Jr C, Ito K, Haser W, Ishida I, Nakanishi N . Intestinal intraepithelial lymphocytes are a distinct set of gamma delta T cells. Nature. 1988; 336(6198):479-81. DOI: 10.1038/336479a0. View

Groh V, Porcelli S, Fabbi M, Lanier L, Picker L, Anderson T . Human lymphocytes bearing T cell receptor gamma/delta are phenotypically diverse and evenly distributed throughout the lymphoid system. J Exp Med. 1989; 169(4):1277-94. PMC: 2189233. DOI: 10.1084/jem.169.4.1277. View

Tolosa E, Roura C, Marti M, Belfiore A, Pujol-Borrell R . Induction of intercellular adhesion molecule-1 but not of lymphocyte function-associated antigen-3 in thyroid follicular cells. J Autoimmun. 1992; 5(1):119-35. DOI: 10.1016/s0896-8411(05)80056-3. View

10.

Lang F, Schatz D, Pollock B, Riley W, Maclaren N, Dumont-Driscoll M . Increased T lymphocytes bearing the gamma-delta T cell receptor in subjects at high risk for insulin dependent diabetes. J Autoimmun. 1991; 4(6):925-33. DOI: 10.1016/0896-8411(91)90055-h. View

11.

Miyazaki A, Mirakian R, Bottazzo G . Adhesion molecule expression in Graves' thyroid glands; potential relevance of granule membrane protein (GMP-140) and intercellular adhesion molecule-1 (ICAM-1) in the homing and antigen presentation processes. Clin Exp Immunol. 1992; 89(1):52-7. PMC: 1554413. DOI: 10.1111/j.1365-2249.1992.tb06876.x. View

12.

Kozbor D, Trinchieri G, Monos D, Isobe M, Russo G, Haney J . Human TCR-gamma+/delta+, CD8+ T lymphocytes recognize tetanus toxoid in an MHC-restricted fashion. J Exp Med. 1989; 169(5):1847-51. PMC: 2189297. DOI: 10.1084/jem.169.5.1847. View

13.

Janis E, Kaufmann S, Schwartz R, Pardoll D . Activation of gamma delta T cells in the primary immune response to Mycobacterium tuberculosis. Science. 1989; 244(4905):713-6. DOI: 10.1126/science.2524098. View

14.

Brennan F, Londei M, Jackson A, Hercend T, Brenner M, Maini R . T cells expressing gamma delta chain receptors in rheumatoid arthritis. J Autoimmun. 1988; 1(4):319-26. DOI: 10.1016/0896-8411(88)90002-9. View

15.

Weetman A, Cohen S, Makgoba M, Borysiewicz L . Expression of an intercellular adhesion molecule, ICAM-1, by human thyroid cells. J Endocrinol. 1989; 122(1):185-91. DOI: 10.1677/joe.0.1220185. View

16.

Matis L, Fry A, Cron R, Cotterman M, Dick R, Bluestone J . Structure and specificity of a class II MHC alloreactive gamma delta T cell receptor heterodimer. Science. 1989; 245(4919):746-9. DOI: 10.1126/science.2528206. View

17.

Goodman T, Lefrancois L . Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J Exp Med. 1989; 170(5):1569-81. PMC: 2189511. DOI: 10.1084/jem.170.5.1569. View

18.

Halstensen T, Scott H, Brandtzaeg P . Intraepithelial T cells of the TcR gamma/delta+ CD8- and V delta 1/J delta 1+ phenotypes are increased in coeliac disease. Scand J Immunol. 1989; 30(6):665-72. DOI: 10.1111/j.1365-3083.1989.tb02474.x. View

19.

Ross D, Cohen A . Acute withdrawal of short-term or prolonged L-triiodothyronine administration to thyroidectomized rats results in similar rapid increases in TSH beta mRNA. J Endocrinol Invest. 1990; 13(2):177-80. DOI: 10.1007/BF03349534. View

20.

Teng W, Cohen S, Posnett D, Weetman A . T cell receptor phenotypes in autoimmune thyroid disease. J Endocrinol Invest. 1990; 13(4):339-42. DOI: 10.1007/BF03349575. View