Increased Epithelial Expression of HLA-DQ and HLA-DP Molecules in Salivary Glands from Patients with Sjögren's Syndrome Compared with Obstructive Sialadenitis

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1993 May 1

PMID 8485911

Citations 10

Authors

P S Thrane

T S Halstensen

H R Haanaes

P Brandtzaeg

Affiliations

Soon will be listed here.

Abstract

Salivary gland specimens from 10 patients with primary Sjögren's syndrome (pSS) were examined by two-colour immunofluorescence with various combinations of monoclonal and polyclonal antibody reagents of the following specificities: human leucocyte antigen (HLA) class I and II (DR, DP and DQ), CD3, CD45 (leucocyte common antigen), various cytokeratins, and factor VIII-related antigen. Tissue specimens from 10 normal glands and 10 glands with obstructive sialadenitis (no known autoimmunity) served as controls. Only some intercalated ducts and scattered acini of the normal major glands expressed HLA class II determinants (< 5% of total epithelial area); the relative proportion of positive elements indicated differential expression (DR > DP > DQ). SS glands contained substantial T cell infiltrates and increased numbers of activated (DR+) T cells; adjacent epithelium showed extensive differential expression of HLA class II determinants (DR > DP > DQ). Glands with obstructive sialadenitis showed similarly increased epithelial expression of HLA-DR but with surprisingly small amounts of concomitant HLA-DP and -DQ expression. Epithelial HLA class II expression probably depends on cytokines as an inductive event, which is not unique for SS but particularly prominent in this disorder. Our results suggest that epithelial expression of HLA-DP or -DQ, rather than -DR, might be a prerequisite for the autoimmune process of SS to develop in genetically susceptible individuals.

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References

Brandtzaeg P . Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol-fixation technique. Immunology. 1974; 26(6):1101-14. PMC: 1423367. View

Altmann D, Sansom D, Marsh S . What is the basis for HLA-DQ associations with autoimmune disease?. Immunol Today. 1991; 12(8):267-70. DOI: 10.1016/0167-5699(91)90124-C. View

Adamson 3rd T, Fox R, Frisman D, Howell F . Immunohistologic analysis of lymphoid infiltrates in primary Sjogren's syndrome using monoclonal antibodies. J Immunol. 1983; 130(1):203-8. View

Hardcastle J, Farrands P, Balfour T, Chamberlain J, Amar S, Sheldon M . Controlled trial of faecal occult blood testing in the detection of colorectal cancer. Lancet. 1983; 2(8340):1-4. DOI: 10.1016/s0140-6736(83)90001-6. View

Brandtzaeg P, Rognum T . Evaluation of tissue preparation methods and paired immunofluorescence staining for immunocytochemistry of lymphomas. Histochem J. 1983; 15(7):655-89. DOI: 10.1007/BF01002987. View

Daniels T . Labial salivary gland biopsy in Sjögren's syndrome. Assessment as a diagnostic criterion in 362 suspected cases. Arthritis Rheum. 1984; 27(2):147-56. DOI: 10.1002/art.1780270205. View

Isenberg D, Rowe D, Tookman A, Hopp A, Griffiths M, Paice E . An immunohistological study of secondary Sjögren's syndrome. Ann Rheum Dis. 1984; 43(3):470-6. PMC: 1001372. DOI: 10.1136/ard.43.3.470. View

Daar A, Fuggle S, Fabre J, Ting A, Morris P . The detailed distribution of MHC Class II antigens in normal human organs. Transplantation. 1984; 38(3):293-8. DOI: 10.1097/00007890-198409000-00019. View

Fox R, Adamson 3rd T, Fong S, Young C, Howell F . Characterization of the phenotype and function of lymphocytes infiltrating the salivary gland in patients with primary Sjogren syndrome. Diagn Immunol. 1983; 1(3):233-9. View

10.

Londei M, Lamb J, Bottazzo G, Feldmann M . Epithelial cells expressing aberrant MHC class II determinants can present antigen to cloned human T cells. Nature. 1984; 312(5995):639-41. DOI: 10.1038/312639a0. View

11.

Lindahl G, Hedfors E, Klareskog L, Forsum U . Epithelial HLA-DR expression and T lymphocyte subsets in salivary glands in Sjögren's syndrome. Clin Exp Immunol. 1985; 61(3):475-82. PMC: 1577280. View

12.

Huitfeldt H, Brandtzaeg P . Various keratin antibodies produce immunohistochemical staining of human myocardium and myometrium. Histochemistry. 1985; 83(5):381-9. DOI: 10.1007/BF00509196. View

13.

Matsui Y, Shapiro H, SHEEHY M, CHRISTENSON L, Staunton D, Eynon E . Differential expression of T cell differentiation antigens and major histocompatibility antigens on activated T cells during the cell cycle. Eur J Immunol. 1986; 16(3):248-51. DOI: 10.1002/eji.1830160307. View

14.

Harley J, Reichlin M, Arnett F, Alexander E, Bias W, Provost T . Gene interaction at HLA-DQ enhances autoantibody production in primary Sjögren's syndrome. Science. 1986; 232(4754):1145-7. DOI: 10.1126/science.3458307. View

15.

Moutsopoulos H, Hooks J, Chan C, Dalavanga Y, Skopouli F, Detrick B . HLA-DR expression by labial minor salivary gland tissues in Sjögren's syndrome. Ann Rheum Dis. 1986; 45(8):677-83. PMC: 1001967. DOI: 10.1136/ard.45.8.677. View

16.

Pesando J, Graf L, Hoffman P . HLA-DP can be expressed with or without -DR molecules on a malignant B cell line. J Immunol. 1986; 137(6):1932-6. View

17.

Fox R, Bumol T, Fantozzi R, Bone R, Schreiber R . Expression of histocompatibility antigen HLA-DR by salivary gland epithelial cells in Sjögren's syndrome. Arthritis Rheum. 1986; 29(9):1105-11. DOI: 10.1002/art.1780290908. View

18.

Most J, Knapp W, Wick G . Class II antigens in Hashimoto thyroiditis. I. Synthesis and expression of HLA-DR and HLA-DQ by thyroid epithelial cells. Clin Immunol Immunopathol. 1986; 41(2):165-74. DOI: 10.1016/0090-1229(86)90100-5. View

19.

Fox R, Pearson G, Vaughan J . Detection of Epstein-Barr virus-associated antigens and DNA in salivary gland biopsies from patients with Sjogren's syndrome. J Immunol. 1986; 137(10):3162-8. View

20.

Foulis A . Class II major histocompatibility complex and organ specific autoimmunity in man. J Pathol. 1986; 150(1):5-11. DOI: 10.1002/path.1711500103. View