Simian Immunodeficiency Virus RNA is Efficiently Encapsidated by Human Immunodeficiency Virus Type 1 Particles

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1993 May 1

PMID 8474168

Citations 75

Authors

T A Rizvi

A T Panganiban

Affiliations

Soon will be listed here.

Abstract

Packaging of retroviral RNA is attained through the specific recognition of a cis-acting encapsidation site (located near the 5' end of the viral RNA) by components of the Gag precursor protein. Human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) are two lentiviruses that lack apparent sequence similarity in their putative encapsidation regions. We used SIV vectors to determine whether HIV-1 particles can recognize the SIV encapsidation site and functionally propagate SIV nucleic acid. SIV nucleic acid was replicated by HIV-1 proteins. Thus, efficient lentivirus pseudotyping can take place at the RNA level. Direct examination of the RNA contents of virus particles indicated that encapsidation of this heterologous RNA is efficient. Characterization of deletion mutants in the untranslated leader region of SIV RNA indicates that only a very short region at the 5' end of the SIV RNA is needed for packaging. Comparison of this region with the corresponding region of HIV-1 reveals that both are marked by secondary structures that are likely to be similar. Thus, it is likely that a similar higher-order RNA structure is required for encapsidation.

Citing Articles

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Initiation of HIV-1 Gag lattice assembly is required for recognition of the viral genome packaging signal.

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