Polypeptide Synthesis Induced in Nicotiana Clevelandii Protoplasts by Infection with Raspberry Ringspot Nepovirus

Overview

Journal Arch Virol

Specialty Microbiology

Date 1993 Jan 1

PMID 8470949

Authors

O Acosta

M A Mayo

Affiliations

Soon will be listed here.

Abstract

Infection of Nicotiana clevelandii protoplasts by raspberry ringspot nepovirus resulted in the accumulation of about 24 polypeptides that differed in M(r) and pI from polypeptides accumulating in mock-inoculated protoplasts. Similar polypeptides accumulated in protoplasts infected with the S and E strains of RRV but different infection-specific polypeptides were detected in protoplasts infected with tobacco ringspot nepovirus. The M(r) of RRV-specific polypeptides ranged from 210,000 to 18,000 and most are presumed to be derived from others by proteolytic cleavage. No evidence was found for marked changes in polypeptide abundance with time after inoculation or for any virus-specific polypeptide becoming disproportionately abundant in the medium during culture.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Blok V, Wardell J, Jolly C, Manoukian A, Robinson D, EDWARDS M . The nucleotide sequence of RNA-2 of raspberry ringspot nepovirus. J Gen Virol. 1992; 73 ( Pt 9):2189-94. DOI: 10.1099/0022-1317-73-9-2189. View

Acosta O, Mayo M . Unusual electrophoretic properties of the coat protein of raspberry ringspot nepovirus. Intervirology. 1990; 31(1):31-7. DOI: 10.1159/000150131. View

Goldbach R, Rezelman G . Orientation of the cleavage map of the 200-kilodalton polypeptide encoded by the bottom-component RNA of cowpea mosaic virus. J Virol. 1983; 46(2):614-9. PMC: 255164. DOI: 10.1128/JVI.46.2.614-619.1983. View

Demangeat G, Hemmer O, Fritsch C, Gall O, Candresse T . In vitro processing of the RNA-2-encoded polyprotein of two nepoviruses: tomato black ring virus and grapevine chrome mosaic virus. J Gen Virol. 1991; 72 ( Pt 2):247-52. DOI: 10.1099/0022-1317-72-2-247. View

Demangeat G, Greif C, Hemmer O, Fritsch C . Analysis of the in vitro cleavage products of the tomato black ring virus RNA-1-encoded 250K polyprotein. J Gen Virol. 1990; 71 ( Pt 8):1649-54. DOI: 10.1099/0022-1317-71-8-1649. View

Wellink J, Jaegle M, Goldbach R . Detection of a Novel Protein Encoded by the Bottom-Component RNA of Cowpea Mosaic Virus, Using Antibodies Raised against a Synthetic Peptide. J Virol. 1987; 61(1):236-8. PMC: 255253. DOI: 10.1128/JVI.61.1.236-238.1987. View

des Francs C, Thiellement H, de Vienne D . Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis: Protease Action as Exemplified by Ribulose Bisphosphate Carboxylase/ Oxygenase Degradation and Procedure to Avoid Proteolysis during Extraction. Plant Physiol. 1985; 78(1):178-82. PMC: 1064698. DOI: 10.1104/pp.78.1.178. View

Vlak J, Smith G, Summers M . Hybridization Selection and In Vitro Translation of Autographa californica Nuclear Polyhedrosis Virus mRNA. J Virol. 1981; 40(3):762-71. PMC: 256688. DOI: 10.1128/JVI.40.3.762-771.1981. View

10.

AMES G, NIKAIDO K . Two-dimensional gel electrophoresis of membrane proteins. Biochemistry. 1976; 15(3):616-23. DOI: 10.1021/bi00648a026. View

11.

Demangeat G, Hemmer O, Reinbolt J, Mayo M, Fritsch C . Virus-specific proteins in cells infected with tomato black ring nepovirus: evidence for proteolytic processing in vivo. J Gen Virol. 1992; 73 ( Pt 7):1609-14. DOI: 10.1099/0022-1317-73-7-1609. View

12.

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

13.

Duncan R, McConkey E . How many proteins are there in a typical mammalian cell?. Clin Chem. 1982; 28(4 Pt 2):749-55. View