Tissue Variation in Hydrocarbon Composition in the Rabbit

Overview

Journal Lipids

Specialty Biochemistry

Date 1977 Feb 1

PMID 846304

Citations 2

Authors

A Ferretti

V P Flanagan

Affiliations

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Abstract

This study, which deals with the distribution of hydrocarbons in seven types of rabbit tissues, was done for the purpose of providing information that might help shed light on the biological relevance of the hydrocarbons in mammalian metabolism. Liver, kidneys, brain, spleen, skeletal muscle, perinephric adipose, and a sample of blood serum were collected from a single animal for analysis of their hydrocarbon composition. The analytical methodology consisted of solvent extraction, saponification (adipose), elution chromatography on hydrated alumina, and combined gas chromatography-mass spectrometry. Hydrocarbons were detected in all of the tissues examined at concentrations estimated to range from 0.1 to 0.01% of the total lipid extracted Three quite distinct distribution modes were recognized. The bulk of the identified components consisted of normal, saturated, nonterpenoid hydrocarbons in the C16 to C33 range. Squalene, phytene, phytadiene, and pristane were the only terpenoids detected. Nonterpenoid branched (iso and anteiso) hydrocarbons were identified unequivocally and in significant amounts in the muscle only. The adipose was the only tissue which was relatively rich in monoalkenes, and its overall hydrocarbon composition closely resembled that of the feed. The results of the study are not consistent with metabolic inertness. The observed qualitative and quantitative differences might reflect function and metabolic activities of the individual organs in a way yet to be elucidated.

Citing Articles

Changes in the DNA of lymphocytes from pristane treated rats.

Garrett L, Bost K, Buttke T, Cuchens M Agents Actions. 1987; 20(1-2):104-12.

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Distribution of hydrocarbons in bovine tissues.

Lintas C, Balduzzi A, Bernardini M, Di Muccio A Lipids. 1979; 14(3):298-303.

PMID: 449633 DOI: 10.1007/BF02533918.

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