Islet Amyloid Polypeptide in the Rabbit and European Hare: Studies on Its Relationship to Amyloidogenesis

Overview

Journal Diabetologia

Specialty Endocrinology

Date 1993 Mar 1

PMID 8462765

Citations 5

Authors

L Christmanson

C Betsholtz

A Leckstrom

U Engstrom

C Cortie

K H Johnson

T E Adrian

P Westermark

Affiliations

Soon will be listed here.

Abstract

In this study, we determined the cDNA-predicted amino acid sequence of positions 9-31 of islet amyloid polypeptide from the rabbit and European hare. A synthetic rabbit/hare islet amyloid polypeptide 20-29 peptide was subsequently shown to be strongly fibrillogenic in vitro even though the putative amyloidogenic AILS sequence at positions 25-28 of human and cat islet amyloid polypeptide is modified in the rabbit and hare by a substitution of phenylalanine for leucine at position 27 (i.e. AIFS). Although islet amyloid polypeptide of both the rabbit and hare has an amyloidogenic sequence and is in fact amyloidogenic in vitro, the apparent lack of in vivo islet amyloidosis in rabbits and hares may be related to relatively low levels of islet amyloid polypeptide production by the islet beta cells in these species. This was supported by our findings that there is no substantial immunoreactivity in either rabbit or hare islets, and no measurable amount either in extracts of rabbit pancreases, or in rabbit plasma. This study supports the need for at least two prerequisites for the development of islet amyloidosis in vivo: an inherent fibrillogenic sequence within the islet amyloid polypeptide molecule and an adequate local concentration of islet amyloid polypeptide to promote self aggregation and formation of islet amyloid.

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References

Nishi M, Sanke T, Nagamatsu S, Bell G, Steiner D . Islet amyloid polypeptide. A new beta cell secretory product related to islet amyloid deposits. J Biol Chem. 1990; 265(8):4173-6. View

Zschiesche W, Jakob W . Pathology of animal amyloidoses. Pharmacol Ther. 1989; 41(1-2):49-83. DOI: 10.1016/0163-7258(89)90102-2. View

Ohagi S, Nishi M, Bell G, ENSINCK J, Steiner D . Sequences of islet amyloid polypeptide precursors of an Old World monkey, the pig-tailed macaque (Macaca nemestrina), and the dog (Canis familiaris). Diabetologia. 1991; 34(8):555-8. DOI: 10.1007/BF00400272. View

Betsholtz C, Christmanson L, Engstrom U, Rorsman F, Jordan K, OBrien T . Structure of cat islet amyloid polypeptide and identification of amino acid residues of potential significance for islet amyloid formation. Diabetes. 1990; 39(1):118-22. DOI: 10.2337/diacare.39.1.118. View

Nishi M, Chan S, Nagamatsu S, Bell G, Steiner D . Conservation of the sequence of islet amyloid polypeptide in five mammals is consistent with its putative role as an islet hormone. Proc Natl Acad Sci U S A. 1989; 86(15):5738-42. PMC: 297705. DOI: 10.1073/pnas.86.15.5738. View

OBrien T, Westermark P, Johnson K . Islet amyloid polypeptide and calcitonin gene-related peptide immunoreactivity in amyloid and tumor cells of canine pancreatic endocrine tumors. Vet Pathol. 1990; 27(3):194-8. DOI: 10.1177/030098589002700307. View

Asai J, Nakazato M, Kangawa K, Matsukura S, Matsuo H . Isolation and sequence determination of rat islet amyloid polypeptide. Biochem Biophys Res Commun. 1989; 164(1):400-5. DOI: 10.1016/0006-291x(89)91733-6. View

Ofverstedt L, Hammarstrom K, Balgobin N, Hjerten S, Pettersson U, Chattopadhyaya J . Rapid and quantitative recovery of DNA fragments from gels by displacement electrophoresis (isotachophoresis). Biochim Biophys Acta. 1984; 782(2):120-6. DOI: 10.1016/0167-4781(84)90014-9. View

Westermark P, Engstrom U, Johnson K, Westermark G, Betsholtz C . Islet amyloid polypeptide: pinpointing amino acid residues linked to amyloid fibril formation. Proc Natl Acad Sci U S A. 1990; 87(13):5036-40. PMC: 54256. DOI: 10.1073/pnas.87.13.5036. View

10.

Clark A, Edwards C, Ostle L, Sutton R, Rothbard J, Morris J . Localisation of islet amyloid peptide in lipofuscin bodies and secretory granules of human B-cells and in islets of type-2 diabetic subjects. Cell Tissue Res. 1989; 257(1):179-85. DOI: 10.1007/BF00221649. View

11.

Cooper G, Willis A, Clark A, Turner R, Sim R, Reid K . Purification and characterization of a peptide from amyloid-rich pancreases of type 2 diabetic patients. Proc Natl Acad Sci U S A. 1987; 84(23):8628-32. PMC: 299599. DOI: 10.1073/pnas.84.23.8628. View

12.

Johnson K, Wernstedt C, OBrien T, Westermark P . Amyloid in the pancreatic islets of the cougar (Felis concolor) is derived from islet amyloid polypeptide (IAPP). Comp Biochem Physiol B. 1991; 98(1):115-9. DOI: 10.1016/0305-0491(91)90316-6. View

13.

Auffray C, Rougeon F . Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980; 107(2):303-14. DOI: 10.1111/j.1432-1033.1980.tb06030.x. View

14.

Johnson K, OBrien T, Jordan K, Westermark P . Impaired glucose tolerance is associated with increased islet amyloid polypeptide (IAPP) immunoreactivity in pancreatic beta cells. Am J Pathol. 1989; 135(2):245-50. PMC: 1879920. View

15.

Westermark P, Wernstedt C, Wilander E, Sletten K . A novel peptide in the calcitonin gene related peptide family as an amyloid fibril protein in the endocrine pancreas. Biochem Biophys Res Commun. 1986; 140(3):827-31. DOI: 10.1016/0006-291x(86)90708-4. View

16.

Jordan K, Murtaugh M, OBrien T, Westermark P, Betsholtz C, Johnson K . Canine IAPP cDNA sequence provides important clues regarding diabetogenesis and amyloidogenesis in type 2 diabetes. Biochem Biophys Res Commun. 1990; 169(2):502-8. DOI: 10.1016/0006-291x(90)90359-u. View

17.

Betsholtz C, Svensson V, Rorsman F, Engstrom U, Westermark G, Wilander E . Islet amyloid polypeptide (IAPP):cDNA cloning and identification of an amyloidogenic region associated with the species-specific occurrence of age-related diabetes mellitus. Exp Cell Res. 1989; 183(2):484-93. DOI: 10.1016/0014-4827(89)90407-2. View

18.

Leffert J, Newgard C, Okamoto H, Milburn J, Luskey K . Rat amylin: cloning and tissue-specific expression in pancreatic islets. Proc Natl Acad Sci U S A. 1989; 86(9):3127-30. PMC: 287078. DOI: 10.1073/pnas.86.9.3127. View

19.

Ogawa A, Harris V, McCorkle S, Unger R, Luskey K . Amylin secretion from the rat pancreas and its selective loss after streptozotocin treatment. J Clin Invest. 1990; 85(3):973-6. PMC: 296519. DOI: 10.1172/JCI114528. View

20.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View