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N.m.r. Lipid Profiles of Cells, Tissues and Body Fluids. Neutral, Non-acidic and Acidic Phospholipid Analysis of Bond Elut Chromatographic Fractions

Overview
Journal Biochem J
Specialty Biochemistry
Date 1993 Mar 15
PMID 8457199
Citations 5
Authors
G T Choi
M Casu
W A Gibbons
Affiliations
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Abstract

Although the advantages of in vitro n.m.r. analysis of cellular lipids have been documented, this purely n.m.r. approach also has drawbacks. Rapid and quantitative separation of total lipids into neutral lipids, non-esterified fatty acids, non-acidic phospholipids and acidic phospholipids using Bond Elut ion-exchange columns as demonstrated here permitted a more quantitative and complete n.m.r. analysis of glycerides, cholesterol, saturated and unsaturated sphingolipids and ether lipids, as well as of diacylcholine and ethanolamine lipids. Acidic lipids were also analysed. The fatty acid compositions of the intact lipids in each of the four Bond Elut fractions were determined from the n.m.r. spectrum of each fraction.

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References
1.
Kaluzny M, Duncan L, Merritt M, Epps D . Rapid separation of lipid classes in high yield and purity using bonded phase columns. J Lipid Res. 1985; 26(1):135-40. View
2.
Christie W . Rapid separation and quantification of lipid classes by high performance liquid chromatography and mass (light-scattering) detection. J Lipid Res. 1985; 26(4):507-12. View
3.
Bandi Z, Reynolds E . Adsorption and reversed-phase high-performance liquid chromatography of p-nitrobenzyl esters of monohydroxy fatty acids. J Chromatogr. 1985; 329(1):57-63. DOI: 10.1016/s0021-9673(01)81895-1. View
4.
Sze D, Jardetzky O . Characterization of lipid composition in stimulated human lymphocytes by 1H-NMR. Biochim Biophys Acta. 1990; 1054(2):198-206. DOI: 10.1016/0167-4889(90)90241-5. View
5.
Axelson M, Mork B, Sjovall J . Occurrence of 3 beta-hydroxy-5-cholestenoic acid, 3 beta,7 alpha-dihydroxy-5-cholestenoic acid, and 7 alpha-hydroxy-3-oxo-4-cholestenoic acid as normal constituents in human blood. J Lipid Res. 1988; 29(5):629-41. View