Akaline Phosphatase: Activity and Variation in Human Diploid Fibroblasts

Overview

Journal Arch Int Physiol Biochim

Specialties Biochemistry
Physiology

Date 1978 Oct 1

PMID 84560

Citations 2

Authors

F J Vanneuville

J G Leroy

A F Van Elsen

Affiliations

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Abstract

A method is introduced for the assay of alkaline phosphatase in homogenates of cultured human skin fibroblasts. In a first group of 11 strains, a four- to fifteen-fold increase of enzyme activity is consistently observed following a period of starvation. In the remaining 31 cell-strains similar specific activities of alkaline phosphatase are found irrespective of medium changes. In regularly fed cultures, an inverse exponential correlation between the specific activity of alkaline phosphatase and the age of the donor has been detected.

Citing Articles

Infantile hypophosphatasia: enzymatic defect explored with alkaline phosphatase-deficient skin fibroblasts in culture.

Whyte M, Rettinger S, Vrabel L Calcif Tissue Int. 1987; 40(5):244-52.

PMID: 3107777 DOI: 10.1007/BF02555256.

Analysis of liver/bone/kidney alkaline phosphatase mRNA, DNA, and enzymatic activity in cultured skin fibroblasts from 14 unrelated patients with severe hypophosphatasia.

Weiss M, Ray K, Fallon M, Whyte M, Fedde K, Lafferty M Am J Hum Genet. 1989; 44(5):686-94.

PMID: 2705456 PMC: 1715639.