1,2-alpha-D-mannosidase from Penicillium Citrinum: Molecular and Enzymic Properties of Two Isoenzymes

Overview

Journal Biochem J

Specialty Biochemistry

Date 1993 Mar 1

PMID 8452520

Citations 6

Authors

T Yoshida

T Inoue

E ICHISHIMA

Affiliations

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Abstract

Two isoforms of acidic 1,2-alpha-D-mannosidases have been isolated from culture filtrate of Penicillium citrinum. The pI values of the two forms, designated 1,2-alpha-mannosidase Ia and Ib, were 4.6 and 4.7 respectively. Isoenzymes Ia and Ib exhibited the same molecular mass which was determined to be 53 kDa by SDS/PAGE and 54 kDa by gel-permeation chromatography. Enzymes Ia and Ib hydrolysed yeast mannan and 1,2-alpha-linked mannooligosaccharides, but did not hydrolyse p-nitrophenyl alpha-D-mannoside. The optimal pH for the hydrolysis of Man(alpha 1-->2)Man was 5.0 for both isoenzymes. Similar kinetic parameters were determined for the two forms. Activation energy was a little lower for Ia than Ib. There was little difference between the enzymes with regard to their performance at acidic or alkaline pH. The N-terminal amino acid sequences of the two enzymes were identical. Analysis of C-terminal peptides, which were prepared by tryptic digestion and anhydrotrypsin-agarose chromatography, showed that Ia and Ib had the same amino acid sequences in the C-terminal region. Tryptic digestion revealed a slight difference between the isoenzymes in the pattern of cleaved peptides on SDS/PAGE.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Forsee W, Palmer C, Schutzbach J . Purification and characterization of an alpha-1,2-mannosidase involved in processing asparagine-linked oligosaccharides. J Biol Chem. 1989; 264(7):3869-76. View

Burditt L, Phillips N, Robinson D, Winchester B, Jolly R . Characterization of the mutant alpha-mannosidase in bovine mannosidosis. Biochem J. 1978; 175(3):1013-22. PMC: 1186164. DOI: 10.1042/bj1751013. View

Ishii S, Yokosawa H, Shiba S, Kasai K . Specific isolation of biologically-active peptides by means of immobilized anhydrotrypsin and anhydrochymotrypsin. Adv Exp Med Biol. 1979; 120A:15-27. DOI: 10.1007/978-1-4757-0926-1_3. View

Davis B . DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964; 121:404-27. DOI: 10.1111/j.1749-6632.1964.tb14213.x. View

SMOGYI M . Notes on sugar determination. J Biol Chem. 1952; 195(1):19-23. View

Norden N, Lundblad A, OCKERMAN P, Jolly R . Mannosidosis in Angus cattle: partial characterization of two mannose containing oligosaccharides. FEBS Lett. 1973; 35(2):209-12. DOI: 10.1016/0014-5793(73)80286-8. View

Tulsiani D, Harris T, Touster O . Swainsonine inhibits the biosynthesis of complex glycoproteins by inhibition of Golgi mannosidase II. J Biol Chem. 1982; 257(14):7936-9. View

Matta K, BAHL O . Glycosidases of Aspergillus niger. IV. Purification and characterization of -mannosidase. J Biol Chem. 1972; 247(6):1780-7. View

10.

Chappell J, GREVILLE G . Effect of silver ions on mitochondrial adenosine triphosphatase. Nature. 1954; 174(4437):930-1. DOI: 10.1038/174930b0. View

11.

Bischoff J, KORNFELD R . The soluble form of rat liver alpha-mannosidase is immunologically related to the endoplasmic reticulum membrane alpha-mannosidase. J Biol Chem. 1986; 261(10):4758-65. View

12.

Stewart T, Mendershausen P, Ballou C . Preparation of a mannopentaose, mannohexaose, and mannoheptaose from Saccharomyces cerevisiae mannan. Biochemistry. 1968; 7(5):1843-54. DOI: 10.1021/bi00845a032. View

13.

Yoshihisa T, Ohsumi Y, Anraku Y . Solubilization and purification of alpha-mannosidase, a marker enzyme of vacuolar membranes in Saccharomyces cerevisiae. J Biol Chem. 1988; 263(11):5158-63. View

14.

Garfin D . Isoelectric focusing. Methods Enzymol. 1990; 182:459-77. DOI: 10.1016/0076-6879(90)82037-3. View

15.

Bischoff J, Liscum L, KORNFELD R . The use of 1-deoxymannojirimycin to evaluate the role of various alpha-mannosidases in oligosaccharide processing in intact cells. J Biol Chem. 1986; 261(10):4766-74. View

16.

Elbein A . Inhibitors of the biosynthesis and processing of N-linked oligosaccharide chains. Annu Rev Biochem. 1987; 56:497-534. DOI: 10.1146/annurev.bi.56.070187.002433. View

17.

ICHISHIMA E, Arai M, Shigematsu Y, Kumagai H . Purification of an acidic alpha-D-mannosidase from Aspergillus saitoi and specific cleavage of 1,2-alpha-D-mannosidic linkage in yeast mannan. Biochim Biophys Acta. 1981; 658(1):45-53. DOI: 10.1016/0005-2744(81)90248-5. View

18.

Tulsiani D, Touster O . The purification and characterization of mannosidase IA from rat liver Golgi membranes. J Biol Chem. 1988; 263(11):5408-17. View

19.

Schutzbach J, Forsee W . Calcium ion activation of rabbit liver alpha 1,2-mannosidase. J Biol Chem. 1990; 265(5):2546-9. View

20.

Park J, Johnson M . A submicrodetermination of glucose. J Biol Chem. 1949; 181(1):149-51. View