Aanlysis of the Frequency and Distribution of Sister Chromatid Exchanges in Cultured Human Lymphocytes

Overview

Journal Hum Genet

Specialty Genetics

Date 1977 Mar 14

PMID 844877

Citations 33

Authors

P E Crossen

M E Drets

F E Arrighi

D A Johnston

Affiliations

Soon will be listed here.

Abstract

Lymphocytes from 20 notmal subjects (11 male and 9 female) were examined for the frequency and location of sister chromatid exchanges (SCE) by the BrdU--Giemsa method. The mean frequency of SCE was 6.37 with little significant variation. One subject had a high number of exchanges in chromosome 1 while the remainder showed a random distribution of exchanges between chromosomes. The frequency of exchanges generally increased with chromosome length. However, chromosome 1, 2 and the B group had more exchanges than expected while the E, F and G grous had less than expected. The distribution of exchanges in chromosomes 1, 2 and the B group was non-random with a concentration of exchanges below the centromere and to a lesser extent on the distal portion of the long arm. The majority of exchanges appeared to occur at the junction between the dark and light G bands. It is suggested that the concentration of exchanges may reflect differences in BrdU incorporation along the length of the chromosome.

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References

Dutrillauz B, Fosse A, PRIEUR M, LeJeune J . [Chromatid exchanges in human mitotic cells. BUDR Treatment and bichromatic fluorescence by aridine orange (author's transl)]. Chromosoma. 1974; 48(3):327-40. DOI: 10.1007/BF00326509. View

Ellison J, BARR H . Quinacrine fluorescence of specific chromosome regions. Late replication and high A: T content in Samoaia leonensis. Chromosoma. 1972; 36(4):375-90. DOI: 10.1007/BF00336794. View

Marin G, Prescott D . THE FREQUENCY OF SISTER CHROMATID EXCHANGES FOLLOWING EXPOSURE TO VARYING DOSES OF H3-THYMIDINE OR X-RAYS. J Cell Biol. 1964; 21:159-67. PMC: 2106437. DOI: 10.1083/jcb.21.2.159. View

Brewen J, Peacock W . The effect of tritiated thymidine on sister-chromatid exchange in a ring chromosome. Mutat Res. 1969; 7(3):433-40. DOI: 10.1016/0027-5107(69)90114-6. View

LATT S . Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc Natl Acad Sci U S A. 1973; 70(12):3395-9. PMC: 427244. DOI: 10.1073/pnas.70.12.3395. View

Galloway S, Evans H . Sister chromatid exchange in human chromosomes from normal individuals and patients with ataxia telangiectasia. Cytogenet Cell Genet. 1975; 15(1):17-29. DOI: 10.1159/000130495. View

Wolff S, Perry P . Differential Giemsa staining of sister chromatids and the study of chromatid exchanges without autoradiography. Chromosoma. 1974; 48(4):341-53. DOI: 10.1007/BF00290991. View

Korenberg J, Freedlender E . Giemsa technique for the detection of sister chromatid exchanges. Chromosoma. 1974; 48(4):355-60. DOI: 10.1007/BF00290992. View

Hsu T, Arrighi F, Saunders G . Compositional heterogeneity of human heterochromatin. Proc Natl Acad Sci U S A. 1972; 69(6):1464-6. PMC: 426726. DOI: 10.1073/pnas.69.6.1464. View

10.

Jones K, Corneo G . Location of satellite and homogeneous DNA sequences on human chromosomes. Nat New Biol. 1971; 233(43):268-71. DOI: 10.1038/newbio233268a0. View

11.

Dev V, Warburton D, MILLER O, Miller D, Erlanger B, Beiser S . Consistent pattern of binding of anti-adenosine antibodies to human metaphase chromosomes. Exp Cell Res. 1972; 74(1):288-93. DOI: 10.1016/0014-4827(72)90509-5. View

12.

LATT S . Localization of sister chromatid exchanges in human chromosomes. Science. 1974; 185(4145):74-6. DOI: 10.1126/science.185.4145.74. View

13.

Taylor J . Sister Chromatid Exchanges in Tritium-Labeled Chromosomes. Genetics. 1958; 43(3):515-29. PMC: 1209899. DOI: 10.1093/genetics/43.3.515. View

14.

Weisblum B, de Haseth P . Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythymidylaterich regions in DNA. Proc Natl Acad Sci U S A. 1972; 69(3):629-32. PMC: 426522. DOI: 10.1073/pnas.69.3.629. View

15.

Carrano A, Wolff S . Distribution of sister chromatid exchanges in the euchromatin and heterochromatin of the Indian muntjac. Chromosoma. 1975; 53(4):361-9. DOI: 10.1007/BF00294083. View

16.

Gibson D, Prescott D . Frequency and sites of sister chromatid exchanges in rat kangaroo chromosomes. Exp Cell Res. 1974; 86(1):209-14. DOI: 10.1016/0014-4827(74)90676-4. View

17.

Chaganti R, Schonberg S, German J . A manyfold increase in sister chromatid exchanges in Bloom's syndrome lymphocytes. Proc Natl Acad Sci U S A. 1974; 71(11):4508-12. PMC: 433916. DOI: 10.1073/pnas.71.11.4508. View

18.

Sperling K, Wegner R, Riehm H, Obe G . Frequency and distribution of sister-chromatid exchanges in a case of Fanconi's anemia. Humangenetik. 1975; 27(3):227-30. DOI: 10.1007/BF00278349. View

19.

Perry P, Wolff S . New Giemsa method for the differential staining of sister chromatids. Nature. 1974; 251(5471):156-8. DOI: 10.1038/251156a0. View

20.

Gibson D, Prescott D . Induction of sister chromatid exchanges in chromosomes of rat kangaroo cells by tritium incorporated into DNA. Exp Cell Res. 1972; 74(2):397-402. DOI: 10.1016/0014-4827(72)90393-x. View