Adipose Pyruvate Carboxylase: Amino Acid Sequence and Domain Structure Deduced from CDNA Sequencing

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1993 Mar 1

PMID 8446588

Citations 11

Authors

J Zhang

W L Xia

K Brew

F Ahmad

Affiliations

Soon will be listed here.

Abstract

The complete amino acid sequence of 3T3-L1 adipocyte pyruvate carboxylase (PC) [pyruvate:carbon-dioxide ligase (ADP-forming), EC 6.4.1.1] has been deduced from sequencing overlapping cDNA clones obtained from an adipocyte cDNA library constructed in the lambda Zap vector. The encoding mRNA for PC promoter contains 4067 nt, including a 3534-nt coding sequence and noncoding regions of 100 and 433 nt at the 5' and 3' ends, respectively. The biotinylated lysine of the encoded PC promoter (1178 amino acids with a calculated M(r) of apocarboxylase = 129,784) is located 35 residues from the COOH-terminal end and, as in most other biotin enzymes, is in the consensus sequence AMKM. The adipocyte PC is closely similar (53% identity) to the yeast enzyme and contains different segments that are homologous with regions from the biotin carboxylase component of Escherichia coli acetyl-CoA carboxylase, the keto acid-binding subunits of Propionibacterium shermanii oxaloacetate transcarboxylase and Klebsiella pneumoniae oxaloacetate decarboxylase, and to the biotin carboxyl-carrier protein of the bacterial biotin enzymes. In addition to the putative mitochondrial targeting signal, functional domains are readily identifiable in the sequence and are in the following order: biotin carboxylase-carboxyltransferase-biotin carboxyl-carrier protein, as proposed for yeast PC.

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References

Schwarz E, Oesterhelt D, Reinke H, Beyreuther K, Dimroth P . The sodium ion translocating oxalacetate decarboxylase of Klebsiella pneumoniae. Sequence of the biotin-containing alpha-subunit and relationship to other biotin-containing enzymes. J Biol Chem. 1988; 263(20):9640-5. View

Li S, Cronan Jr J . The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase. J Biol Chem. 1992; 267(2):855-63. View

Lane M, Moss J, Polakis S . Acetyl coenzyme A carboxylase. Curr Top Cell Regul. 1974; 8(0):139-95. View

UTTER M, Barden R, Taylor B . Pyruvate carboxylase: an evaluation of the relationships between structure and mechanism and between structure and catalytic activity. Adv Enzymol Relat Areas Mol Biol. 1975; 42:1-72. DOI: 10.1002/9780470122877.ch1. View

Mackall J, Student A, Polakis S, Lane M . Induction of lipogenesis during differentiation in a "preadipocyte" cell line. J Biol Chem. 1976; 251(20):6462-4. View

Sutton M, Fall R, NERVI A, ALBERTS A, VAGELOS P, Bradshaw R . Amino acid sequence of Escherichia coli biotin carboxyl carrier protein (9100). J Biol Chem. 1977; 252(11):3934-40. View

Wood H, Barden R . Biotin enzymes. Annu Rev Biochem. 1977; 46:385-413. DOI: 10.1146/annurev.bi.46.070177.002125. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Libor S, Sundaram T, Warwick R, Chapman J, Grundy S . Pyruvate carboxylase from a thermophilic Bacillus: some molecular characteristics. Biochemistry. 1979; 18(17):3647-53. DOI: 10.1021/bi00584a001. View

10.

Freytag S, UTTER M . Induction of pyruvate carboxylase apoenzyme and holoenzyme in 3T3-L1 cells during differentiation. Proc Natl Acad Sci U S A. 1980; 77(3):1321-5. PMC: 348486. DOI: 10.1073/pnas.77.3.1321. View

11.

Angus C, Lane M, Rosenfeld P, Kelly T . Increase in translatable mRNA for mitochondrial pyruvate carboxylase during differentiation of 3T3 preadipocytes. Biochem Biophys Res Commun. 1981; 103(4):1216-22. DOI: 10.1016/0006-291x(81)90252-7. View

12.

Kozak M . Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol Rev. 1983; 47(1):1-45. PMC: 281560. DOI: 10.1128/mr.47.1.1-45.1983. View

13.

Srivastava G, Borthwick I, Brooker J, Wallace J, May B, Elliott W . Hemin inhibits transfer of pre-delta-aminolevulinate synthase into chick embryo liver mitochondria. Biochem Biophys Res Commun. 1983; 117(1):344-9. DOI: 10.1016/0006-291x(83)91582-6. View

14.

Robinson B, Oei J, Sherwood W, Applegarth D, Wong L, Haworth J . The molecular basis for the two different clinical presentations of classical pyruvate carboxylase deficiency. Am J Hum Genet. 1984; 36(2):283-94. PMC: 1684418. View

15.

Attwood P, Keech D . Pyruvate carboxylase. Curr Top Cell Regul. 1984; 23:1-55. DOI: 10.1016/b978-0-12-152823-2.50005-2. View

16.

STERNBERG M, Taylor W . Modelling the ATP-binding site of oncogene products, the epidermal growth factor receptor and related proteins. FEBS Lett. 1984; 175(2):387-92. DOI: 10.1016/0014-5793(84)80774-7. View

17.

Freytag S, Collier K . Molecular cloning of a cDNA for human pyruvate carboxylase. Structural relationship to other biotin-containing carboxylases and regulation of mRNA content in differentiating preadipocytes. J Biol Chem. 1984; 259(20):12831-7. View

18.

Moller W, Amons R . Phosphate-binding sequences in nucleotide-binding proteins. FEBS Lett. 1985; 186(1):1-7. DOI: 10.1016/0014-5793(85)81326-0. View

19.

Ahmad F, Ahmad P, Mendez A . Rat liver pyruvate carboxylase. Purification, detection and quantification of apo and holo forms by immuno-blotting and by an enzyme-linked immunosorbent assay. Biochem J. 1986; 236(2):527-33. PMC: 1146872. DOI: 10.1042/bj2360527. View

20.

Lamhonwah A, Quan F, Gravel R . Sequence homology around the biotin-binding site of human propionyl-CoA carboxylase and pyruvate carboxylase. Arch Biochem Biophys. 1987; 254(2):631-6. DOI: 10.1016/0003-9861(87)90146-9. View