Use of Tetramethylbenzidine in Plasma Hemoglobin Assay

Overview

Journal Clin Chem

Publisher Oxford University Press

Specialty Biochemistry

Date 1977 Jan 1

PMID 844173

Citations 11

Authors

J C Standefer

D Vanderjagt

Affiliations

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Abstract

Plasma hemoglobin can be assayed by substituting 3,3',5,5'-tetramethylbenzidine for benzidine (a carcinogen) as the chromogenic reducing substance. Sensitivity, precision, and accuracy compare well with methods in which benzidine is used. Plasma inhibition can be accommodated by preincubation with hydrogen peroxide.

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