Cloning and Sequencing of a Full Length CDNA Encoding Ovine Lipoprotein Lipase
Overview
Biophysics
Affiliations
A cDNA clone encoding lipoprotein lipase has been isolated from an ovine adipocyte library. Sequencing of this clone has revealed a single open reading frame encoding a 450 amino acid protein. Comparison with known LPL sequences from other species shows a high degree of conservation in the sequence of the protein and in the 5' untranslated region of the DNA sequence.
Cloning of Xuhuai goat lipoprotein lipase gene and the preparation of transgenic sheep.
Qin Y, Zhang Y, Yin Y, Xu F, Gao B, Shi Q Mol Biol Rep. 2012; 39(8):8439-46.
PMID: 22711304 DOI: 10.1007/s11033-012-1697-6.
Holmes R, VandeBerg J, Cox L Comp Biochem Physiol Part D Genomics Proteomics. 2011; 6(2):224-34.
PMID: 21561822 PMC: 3102144. DOI: 10.1016/j.cbd.2011.04.003.
Human lipoprotein lipase last exon is not translated, in contrast to lower vertebrates.
Arnault F, Etienne J, Noe L, Raisonnier A, Brault D, Harney J J Mol Evol. 1996; 43(2):109-15.
PMID: 8660435 DOI: 10.1007/BF02337355.
Soteriou A, Cryer A Biochem J. 1994; 299 ( Pt 2):417-23.
PMID: 8172602 PMC: 1138288. DOI: 10.1042/bj2990417.