Nucleotide Sequence and Regulated Expression of the Salmonella FljA Gene Encoding a Repressor of the Phase 1 Flagellin Gene

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1993 Jan 1

PMID 8437573

Citations 5

Authors

T Hanafusa

K Saito

A Tominaga

M Enomoto

Affiliations

Soon will be listed here.

Abstract

The nucleotide sequence of Salmonella abortus-equi fljA, which together with the phase 2 flagellin gene constitutes the fljBA operon and encodes the repressor for the phase 1 flagellin gene fliC, was determined. The repressor was predicted to be a basic protein consisting of 179 amino acid residues (M(r) = 20419 Da) encoded by ORFII. This was confirmed by the fact that host fliC is repressed by plasmid-encoded ORFII, which indeed expresses a 20 kDa product as determined by urea SDS-polyacrylamide gel electrophoresis. An amino acid sequence capable of forming a helix-turn-helix type of structure was predicted in the C-terminal region of FljA. A rho-independent intercistronic terminator was detected between fljB and fljA. Chloramphenicol acetyltransferase (CAT) assays of fusions indicated that the terminator is capable of reducing expression of fljA to the level of a few percent, relative to fljB in broth cultures and to 1% in M9 glycerol cultures.

Citing Articles

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