Standard System for Infecting Ticks (Acari: Ixodidae) with the Lyme Disease Spirochete, Borrelia Burgdorferi

Overview

Journal J Med Entomol

Publisher Oxford University Press

Specialty Biology

Date 1993 Jan 1

PMID 8433326

Citations 50

Authors

J Piesman

Affiliations

Soon will be listed here.

Abstract

A standard system for infecting ticks with the Lyme disease spirochete Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner is defined. Rodents infected via tick feeding or inoculation of tick homogenates were more infectious to ticks than rodents infected with culture-derived spirochetes. White laboratory mice were more infectious than hamsters. Three strains of B. burgdorferi (JD1, B31, and WI210) produced batches of infected ticks with > 80% infection when mice were infected with tick-derived material. Ixodes dammini Spielman, Clifford, Piesman & Corwin were 3.6x more efficient than I. pacificus Cooley & Kohls in acquiring and maintaining infection with two California strains of B. burgdorferi, originally isolated from I. pacificus.

Citing Articles

Vector competence studies with hard ticks and Borrelia burgdorferi sensu lato spirochetes: A review.

Eisen L Ticks Tick Borne Dis. 2020; 11(3):101359.

PMID: 32067949 PMC: 7127979. DOI: 10.1016/j.ttbdis.2019.101359.

Transmission of the Lyme Disease Spirochete Borrelia mayonii in Relation to Duration of Attachment by Nymphal Ixodes scapularis (Acari: Ixodidae).

Dolan M, Breuner N, Hojgaard A, Boegler K, Hoxmeier J, Replogle A J Med Entomol. 2017; 54(5):1360-1364.

PMID: 28874016 PMC: 5968629. DOI: 10.1093/jme/tjx089.

Metabolomics of the tick-Borrelia interaction during the nymphal tick blood meal.

Hoxmeier J, Fleshman A, Broeckling C, Prenni J, Dolan M, Gage K Sci Rep. 2017; 7:44394.

PMID: 28287618 PMC: 5347386. DOI: 10.1038/srep44394.

Enhanced Protective Immunogenicity of Homodimeric Borrelia burgdorferi Outer Surface Protein C.

Edmondson D, Prabhakaran S, Norris S, Ullmann A, Piesman J, Dolan M Clin Vaccine Immunol. 2016; 24(1).

PMID: 27733423 PMC: 5216437. DOI: 10.1128/CVI.00306-16.

Comparison of Tick Feeding Success and Vector Competence for Borrelia burgdorferi Among Immature Ixodes scapularis (Ixodida: Ixodidae) of Both Southern and Northern Clades.

Goddard J, Embers M, Hojgaard A, Piesman J J Med Entomol. 2015; 52(1):81-5.

PMID: 26336283 PMC: 4817621. DOI: 10.1093/jme/tju005.