Improved Primary Immunodiagnosis of Alveolar Echinococcosis in Humans by an Enzyme-linked Immunosorbent Assay Using the Em2plus Antigen

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1993 Feb 1

PMID 8432825

Citations 42

Authors

B Gottstein

P Jacquier

S Bresson-Hadni

J Eckert

Affiliations

Soon will be listed here.

Abstract

Alveolar echinococcosis (AE) in humans is generally a fatal disease when not diagnosed early enough to provide curative treatment such as radical surgery. Immunodiagnosis for early detection of AE was improved by the isolation of an affinity-purified metacestode Em2 antigen and by the synthesis of recombinant Echinococcus multilocularis antigen II/3-10. Both antigens were individually assessed by enzyme-linked immunosorbent assay (ELISA) and demonstrated high specificities and diagnostic sensitivities, although both missed approximately 4 to 11% of diagnostic cases of AE. To provide an optimal serodiagnostic test, we investigated the two purified antigens by using a test employing a mixture of both purified antigens (designated Em2plus antigen) in one assay. For comparative purposes, crude E. multilocularis and Echinococcus granulosus metacestode antigens were investigated as well. The Em2plus ELISA proved to be the optimal diagnostic test with the highest diagnostic sensitivity, 97%, in serum samples from 140 patients with AE and an overall specificity of 99% for infections due to other Echinococcus and non-Echinococcus parasites. The new test combination (Em2plus ELISA) is suggested for the serodiagnosis of AE in patients and for seroepidemiological surveys.

Citing Articles

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Serological Assays for Alveolar and Cystic Echinococcosis-A Comparative Multi-Test Study in Switzerland and Kyrgyzstan.

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Diagnostic and follow-up performance of serological tests for different forms/courses of alveolar echinococcosis.

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