Adhesion-dependent Protein Tyrosine Phosphorylation in Neutrophils Treated with Tumor Necrosis Factor

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1993 Feb 1

PMID 8425901

Citations 38

Authors

M Fuortes

W W Jin

C Nathan

Affiliations

Soon will be listed here.

Abstract

Human neutrophils (PMN) respond to tumor necrosis factor (TNF) by releasing their granules, reorganizing their cytoskeleton, and massively secreting hydrogen peroxide. This response is dependent on adhesion to extracellular matrix proteins and expression of CD11b/CD18 integrins (Nathan, C., S. Srimal, C. Farber, E. Sanchez, L. Kabbash, A. Asch, J. Gailit, and S. D. Wright. 1989. J. Cell Biol. 109:1341-1349). We investigated the role of tyrosine phosphorylation in the response of PMN to TNF. PMN adherent to protein-coated surfaces but not suspended PMN showed tyrosine phosphorylation of several proteins (approximately 150, approximately 115, approximately 75, and approximately 65 kD) in response to TNF. Tyrosine phosphorylation was evident 5 min after addition of TNF and lasted at least 2 h. The tyrosine kinase inhibitors K252a, genistein and ST638 suppressed tyrosine phosphorylation and blocked hydrogen peroxide production in a reversible manner at low concentrations. Tyrosine kinase inhibitors also blocked the spreading of PMN in response to TNF. Dihydrocytochalasin B did not inhibit tyrosine phosphorylation, but in its presence phosphorylation was rapidly reversed. By immunocytochemistry, the majority of tyrosine phosphoproteins were localized to focal adhesions. Thus TNF-induced tyrosine phosphorylation depends on adhesion of PMN to extracellular matrix proteins, and participates in the transduction of the signals that direct the cells to spread on a biological surface and undergo a respiratory burst.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Badwey J, Erickson R, Curnutte J . Staurosporine inhibits the soluble and membrane-bound protein tyrosine kinases of human neutrophils. Biochem Biophys Res Commun. 1991; 178(2):423-9. DOI: 10.1016/0006-291x(91)90124-p. View

Maher P, Pasquale E, Wang J, Singer S . Phosphotyrosine-containing proteins are concentrated in focal adhesions and intercellular junctions in normal cells. Proc Natl Acad Sci U S A. 1985; 82(19):6576-80. PMC: 391252. DOI: 10.1073/pnas.82.19.6576. View

Kase H, Iwahashi K, Nakanishi S, Matsuda Y, Yamada K, Takahashi M . K-252 compounds, novel and potent inhibitors of protein kinase C and cyclic nucleotide-dependent protein kinases. Biochem Biophys Res Commun. 1987; 142(2):436-40. DOI: 10.1016/0006-291x(87)90293-2. View

Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh N . Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem. 1987; 262(12):5592-5. View

Quintrell N, Lebo R, Varmus H, Bishop J, Pettenati M, Le Beau M . Identification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells. Mol Cell Biol. 1987; 7(6):2267-75. PMC: 365351. DOI: 10.1128/mcb.7.6.2267-2275.1987. View

Kraft A, Berkow R . Tyrosine kinase and phosphotyrosine phosphatase activity in human promyelocytic leukemia cells and human polymorphonuclear leukocytes. Blood. 1987; 70(2):356-62. View

Shiraishi T, Domoto T, Imai N, Shimada Y, Watanabe K . Specific inhibitors of tyrosine-specific protein kinase, synthetic 4-hydroxycinnamamide derivatives. Biochem Biophys Res Commun. 1987; 147(1):322-8. DOI: 10.1016/s0006-291x(87)80124-9. View

Nathan C . Neutrophil activation on biological surfaces. Massive secretion of hydrogen peroxide in response to products of macrophages and lymphocytes. J Clin Invest. 1987; 80(6):1550-60. PMC: 442423. DOI: 10.1172/JCI113241. View

10.

Wolpe S, Davatelis G, Sherry B, Beutler B, Hesse D, Nguyen H . Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties. J Exp Med. 1988; 167(2):570-81. PMC: 2188834. DOI: 10.1084/jem.167.2.570. View

11.

Huang C, Laramee G, Casnellie J . Chemotactic factor induced tyrosine phosphorylation of membrane associated proteins in rabbit peritoneal neutrophils. Biochem Biophys Res Commun. 1988; 151(2):794-801. DOI: 10.1016/s0006-291x(88)80351-6. View

12.

Smith R, Justen J, Sam L . Effects of a protein kinase C inhibitor, K-252a, on human polymorphonuclear neutrophil responsiveness. Biochem Biophys Res Commun. 1988; 152(3):1497-503. DOI: 10.1016/s0006-291x(88)80455-8. View

13.

Altieri D, Bader R, Mannucci P, Edgington T . Oligospecificity of the cellular adhesion receptor Mac-1 encompasses an inducible recognition specificity for fibrinogen. J Cell Biol. 1988; 107(5):1893-900. PMC: 2115324. DOI: 10.1083/jcb.107.5.1893. View

14.

Nathan C . Respiratory burst in adherent human neutrophils: triggering by colony-stimulating factors CSF-GM and CSF-G. Blood. 1989; 73(1):301-6. View

15.

Hamaguchi M, Grandori C, Hanafusa H . Phosphorylation of cellular proteins in Rous sarcoma virus-infected cells: analysis by use of anti-phosphotyrosine antibodies. Mol Cell Biol. 1988; 8(8):3035-42. PMC: 363529. DOI: 10.1128/mcb.8.8.3035-3042.1988. View

16.

Nasmith P, Mills G, Grinstein S . Guanine nucleotides induce tyrosine phosphorylation and activation of the respiratory burst in neutrophils. Biochem J. 1989; 257(3):893-7. PMC: 1135671. DOI: 10.1042/bj2570893. View

17.

Tracey K, Vlassara H, Cerami A . Cachectin/tumour necrosis factor. Lancet. 1989; 1(8647):1122-6. DOI: 10.1016/s0140-6736(89)92394-5. View

18.

Gomez-Cambronero J, Huang C, Bonak V, Wang E, Casnellie J, Shiraishi T . Tyrosine phosphorylation in human neutrophil. Biochem Biophys Res Commun. 1989; 162(3):1478-85. DOI: 10.1016/0006-291x(89)90841-3. View

19.

Berkow R, Dodson R, Kraft A . Human neutrophils contain distinct cytosolic and particulate tyrosine kinase activities: possible role in neutrophil activation. Biochim Biophys Acta. 1989; 997(3):292-301. DOI: 10.1016/0167-4838(89)90200-8. View

20.

Nathan C, Srimal S, Farber C, Sanchez E, Kabbash L, Asch A . Cytokine-induced respiratory burst of human neutrophils: dependence on extracellular matrix proteins and CD11/CD18 integrins. J Cell Biol. 1989; 109(3):1341-9. PMC: 2115779. DOI: 10.1083/jcb.109.3.1341. View