Hyaluronate Synthase: Cloning and Sequencing of the Gene from Streptococcus Sp

Overview

Journal Biochem J

Specialty Biochemistry

Date 1993 Jan 1

PMID 8424756

Citations 12

Authors

M Lansing

S Lellig

A Mausolf

I Martini

F Crescenzi

M ORegan

P Prehm

Affiliations

Soon will be listed here.

Abstract

The complete nucleotide sequence of hyaluronate synthase from Streptococcus sp. and its flanking regions is presented. The gene locus was designated has. Southern-blotting results suggested that the gene was conserved in hyaluronate-producing streptococci. A putative translation-initiation codon was identified and the open reading frame consists of 1566 bp, specifying a protein of 56 kDa. Sequences resembling the promoter and ribosome-binding site of Gram-positive organisms are found upstream of the synthase. The predicted amino-acid sequence reveals the presence of a 35-residue signal peptide. The sequence has some similarity to bacterial peptide-binding proteins.

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