Presence of Protein Constituents of the Gram-positive Bacterial Phosphotransferase Regulatory System in Acholeplasma Laidlawii

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1993 Oct 1

PMID 8407837

Citations 6

Authors

C Hoischen

A Dijkstra

S Rottem

J Reizer

M H Saier Jr

Affiliations

Soon will be listed here.

Abstract

Acholeplasma species have been reported to lack a functional phosphoenolpyruvate:sugar phosphotransferase system (PTS). We show here that Acholeplasma laidlawii possesses activities of enzyme I, HPr, HPr(ser) kinase, and HPr(ser-P) phosphatase but lacks detectable activities of enzymes II of the PTS. HPr from this organism was purified, and the regulatory properties of the kinase and phosphatase were characterized and shown to differ from those of previously studied bacteria. The results suggest the presence of an incomplete PTS in A. laidlawii which has the potential to function in a unique regulatory capacity.

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References

Hengstenberg W, Penberthy W, Hill K, Morse M . Phosphotransferase system of Staphylococcus aureus: its requirement for the accumulation and metabolism of galactosides. J Bacteriol. 1969; 99(2):383-8. PMC: 250027. DOI: 10.1128/jb.99.2.383-388.1969. View

Wright A . Transcriptional regulation of the bgl operon of Escherichia coli involves phosphotransferase system-mediated phosphorylation of a transcriptional antiterminator. J Cell Biochem. 1993; 51(1):83-90. DOI: 10.1002/jcb.240510115. View

CIRILLO V, Razin S . Distribution of a phosphoenolypyruvate-dependent sugar phosphotransferase system in mycoplasms. J Bacteriol. 1973; 113(1):212-7. PMC: 251620. DOI: 10.1128/jb.113.1.212-217.1973. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Saier Jr M, Feucht B, Mora W . Sugar phosphate: sugar transphosphorylation and exchange group translocation catalyzed by the enzyme 11 complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. J Biol Chem. 1977; 252(24):8899-907. View

Saier Jr M, Cox D, Moczydlowski E . Sugar phosphate:sugar transphosphorylation coupled to exchange group translocation catalyzed by the enzyme II complexes of the phosphoenolpyruvate:sugar phosphotransferase system in membrane vesicles of Escherichia coli. J Biol Chem. 1977; 252(24):8908-16. View

Reizer J, Novotny M, Hengstenberg W, Saier Jr M . Properties of ATP-dependent protein kinase from Streptococcus pyogenes that phosphorylates a seryl residue in HPr, a phosphocarrier protein of the phosphotransferase system. J Bacteriol. 1984; 160(1):333-40. PMC: 214721. DOI: 10.1128/jb.160.1.333-340.1984. View

Deutscher J, Kessler U, Hengstenberg W . Streptococcal phosphoenolpyruvate: sugar phosphotransferase system: purification and characterization of a phosphoprotein phosphatase which hydrolyzes the phosphoryl bond in seryl-phosphorylated histidine-containing protein. J Bacteriol. 1985; 163(3):1203-9. PMC: 219260. DOI: 10.1128/jb.163.3.1203-1209.1985. View

Romano A, Brino G, Peterkofsky A, Reizer J . Regulation of beta-galactoside transport and accumulation in heterofermentative lactic acid bacteria. J Bacteriol. 1987; 169(12):5589-96. PMC: 213992. DOI: 10.1128/jb.169.12.5589-5596.1987. View

10.

Reizer J, Peterkofsky A, Romano A . Evidence for the presence of heat-stable protein (HPr) and ATP-dependent HPr kinase in heterofermentative lactobacilli lacking phosphoenolpyruvate:glycose phosphotransferase activity. Proc Natl Acad Sci U S A. 1988; 85(7):2041-5. PMC: 279924. DOI: 10.1073/pnas.85.7.2041. View

11.

Eisermann R, Deutscher J, Hengstenberg W . Site-directed mutagenesis with the ptsH gene of Bacillus subtilis. Isolation and characterization of heat-stable proteins altered at the ATP-dependent regulatory phosphorylation site. J Biol Chem. 1988; 263(32):17050-4. View

12.

Reizer J, Saier Jr M, Deutscher J, Grenier F, Thompson J, Hengstenberg W . The phosphoenolpyruvate:sugar phosphotransferase system in gram-positive bacteria: properties, mechanism, and regulation. Crit Rev Microbiol. 1988; 15(4):297-338. DOI: 10.3109/10408418809104461. View

13.

Reizer J, Sutrina S, Saier M, Stewart G, Peterkofsky A, Reddy P . Mechanistic and physiological consequences of HPr(ser) phosphorylation on the activities of the phosphoenolpyruvate:sugar phosphotransferase system in gram-positive bacteria: studies with site-specific mutants of HPr. EMBO J. 1989; 8(7):2111-20. PMC: 401104. DOI: 10.1002/j.1460-2075.1989.tb03620.x. View

14.

Reizer J . Regulation of sugar uptake and efflux in gram-positive bacteria. FEMS Microbiol Rev. 1989; 5(1-2):149-56. DOI: 10.1016/0168-6445(89)90019-3. View

15.

Meadow N, Fox D, Roseman S . The bacterial phosphoenolpyruvate: glycose phosphotransferase system. Annu Rev Biochem. 1990; 59:497-542. DOI: 10.1146/annurev.bi.59.070190.002433. View

16.

Saier Jr M, Reizer J . Proposed uniform nomenclature for the proteins and protein domains of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. J Bacteriol. 1992; 174(5):1433-8. PMC: 206537. DOI: 10.1128/jb.174.5.1433-1438.1992. View

17.

Reizer J, Sutrina S, Wu L, Deutscher J, Reddy P, Saier Jr M . Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli. J Biol Chem. 1992; 267(13):9158-69. View

18.

Saier Jr M . Introduction: protein phosphorylation and signal transduction in bacteria. J Cell Biochem. 1993; 51(1):1-6. DOI: 10.1002/jcb.240510102. View

19.

Reizer J, Romano A, Deutscher J . The role of phosphorylation of HPr, a phosphocarrier protein of the phosphotransferase system, in the regulation of carbon metabolism in gram-positive bacteria. J Cell Biochem. 1993; 51(1):19-24. DOI: 10.1002/jcb.240510105. View

20.

Stewart G . Catabolite repression in the gram-positive bacteria: generation of negative regulators of transcription. J Cell Biochem. 1993; 51(1):25-8. DOI: 10.1002/jcb.240510106. View